CAJ | 학술논문

目的：探讨应用平阳霉素新辅助化疗对阴茎癌组织基因蛋白c-myc、Ras-P21、P53表达的影响及意义。方法收集哈尔滨医科大学附属肿瘤医院泌尿外科1995～2005年罹患阴茎鳞状细胞癌的住院患者100例，按照术前是否进行辅助化疗分为两组，其中新辅助化疗组50例，术前应用平阳霉素化疗7 d，化疗后行阴茎部分切除术，并行双侧腹股沟淋巴结改良清扫术；对照组50例，直接行手术治疗(手术治疗方法同新辅助化疗组)，术前不进行化学药物治疗。应用免疫组织化学法(SP)对两组患者的阴茎鳞状细胞癌组织进行c-myc、Ras-P21以及P53等指标检测。结果新辅助化疗组组织标本中c-myc、Ras-P21、P53等指标表达的阳性率经检测分别为30豫、27豫、23豫。对照组组织标本中，c-myc、Ras-P21、P53等指标表达的阳性率经检测分别为52豫、48豫、50豫。新辅助化疗组与对照组的c-myc、Ras-P21、P53指标表达阳性率比较，差异有统计学意义(P<0.05)。结论应用平阳霉素新辅助化疗后，阴茎鳞状细胞癌组织中c-myc、Ras-P21、P53蛋白的表达明显下降，平阳霉素新辅助化疗可降低阴茎癌的恶性程度。
목적：탐토응용평양매소신보조화료대음경암조직기인단백c-myc、Ras-P21、P53표체적영향급의의。방법수집합이빈의과대학부속종류의원비뇨외과1995～2005년리환음경린상세포암적주원환자100례，안조술전시부진행보조화료분위량조，기중신보조화료조50례，술전응용평양매소화료7 d，화료후행음경부분절제술，병행쌍측복고구림파결개량청소술；대조조50례，직접행수술치료(수술치료방법동신보조화료조)，술전불진행화학약물치료。응용면역조직화학법(SP)대량조환자적음경린상세포암조직진행c-myc、Ras-P21이급P53등지표검측。결과신보조화료조조직표본중c-myc、Ras-P21、P53등지표표체적양성솔경검측분별위30예、27예、23예。대조조조직표본중，c-myc、Ras-P21、P53등지표표체적양성솔경검측분별위52예、48예、50예。신보조화료조여대조조적c-myc、Ras-P21、P53지표표체양성솔비교，차이유통계학의의(P<0.05)。결론응용평양매소신보조화료후，음경린상세포암조직중c-myc、Ras-P21、P53단백적표체명현하강，평양매소신보조화료가강저음경암적악성정도。
Objective To evaluate the influence and significance of Pingyangmycin neoajuvant chemotherapy on the c-myc, Ras-P21 and P53 protein expression in penile cancer on. Methods The Data of 100 patiens with penile squamous cell carcinoma were obtained in the Department of Urology, the Tumor Hospital of Harbin Medical University, from 1995 to 2005, and they were divided into two groups according to whether given the neoajuvant chemotherapy before the surgery or not. 50 patients in the neoajuvant group were given Pingyangmycin preoperative chemotherapy for 7 days before surgery, after the chemotherapy, partial excision of penis plus improved inguinal lymph node dissection was per-formed. 50 patients in the control group were given partial excision of penis plus improved inguinal lymph node dissec-tion directly without any pre-operative chemotherapy. All pathology specimens were detected of c-myc, Ras-P21, P53 protein product expression by immunohistochemical staining assay. Results In neoajuvant chemotherapy group, the pos-itive expression rates of c-myc, Ras-P21, P53 were 30%, 27%, 23%,respectively. While in control group, the positive expression rates of c-myc, Ras-P21, P53 were 52%, 48%, 50%, the differences were statistically significant (P<0.05). Conclusion The c-myc, Ras-P21, P53 protein expressions are significantly decreased in the tissue of Pingyangmycin neoajuvant chemotherapy of penile cancer, Pingyangmycin neoajuvant chemotherapy may decrease the degree of malig-nancy of penile cancer.