CAJ | 학술논문

目的：探讨血管紧张素I (Angiotensin I ,AngI )及血管紧张素转化酶抑制剂贝那普利对体外培养的肝星状细胞胰岛素生长因子结合蛋白-2（insulin-like growth factor binding protein-2,IGFBP-2)mRNA表达的影响。方法采用肝星状细胞珠HSC-T 6作为研究模型，将培养的肝星状细胞分为对照组、AngI 组、贝那普利组和贝那普利+AngI 组采用实时荧光定量PCR(Realtime fluores-cence quantitative PCR,RTFQ PCR)的方法检测肝星状细胞中IGFBP-2 mRNA的表达水平。结果(1)在体外培养的肝星状细胞中AngI 组高于对照组，差异有统计学意义（P<0.05）；(2)AngI+贝那普利组IGFBP-2 mRNA低于AngI组(P<0.05)。结论 AngI 能促进肝星状细胞IGFBP-2 mRNA表达，贝那普利的抗肝纤维化作用，可能与IGFBP-2 mRNA表达降低有关。
목적：탐토혈관긴장소I (Angiotensin I ,AngI )급혈관긴장소전화매억제제패나보리대체외배양적간성상세포이도소생장인자결합단백-2（insulin-like growth factor binding protein-2,IGFBP-2)mRNA표체적영향。방법채용간성상세포주HSC-T 6작위연구모형，장배양적간성상세포분위대조조、AngI 조、패나보리조화패나보리+AngI 조채용실시형광정량PCR(Realtime fluores-cence quantitative PCR,RTFQ PCR)적방법검측간성상세포중IGFBP-2 mRNA적표체수평。결과(1)재체외배양적간성상세포중AngI 조고우대조조，차이유통계학의의（P<0.05）；(2)AngI+패나보리조IGFBP-2 mRNA저우AngI조(P<0.05)。결론 AngI 능촉진간성상세포IGFBP-2 mRNA표체，패나보리적항간섬유화작용，가능여IGFBP-2 mRNA표체강저유관。
Objective To explore the effects of angiotensin II and angiotensin converting enzyme in-hibitor benazepril on insulin-like growth factor binding protein-2 mRNA (IGFBP-2 mRNA) expression of in vitro cultured hepatic stellate cell (HSC-T 6 ) cells. Methods HSC-T 6 rat hepatic stellate cells line was chosen as the study model. Cultured HSCs were randomized into the control group, AngII group, benazepril group and AngII+benazepril group. The expression of IGFBP-2 mRNA in different group was deteminned by the Realtime fluores-cence quantitative PCR RTFQ PCR meththod. Results Hepatic stellate cells in vitro culture of AngII group is higher than the control group (P<0.05). AngII+benazepril group were reduced than higher than the control group (P<0.05). Conclusion AngII can promote the hepatic stellate cells IGFBP-2 mRNA expression Benazepril decreases the expression IGFBP-2 mRNA in hepaic fibrosis which might inhibit the liver fibrosis.