CAJ | 학술논문

目的：研究茶黄素对原代培养的大鼠气道上皮细胞表达 TLR4及分泌 TNF-α和 IL-6的影响。方法用脂多糖（LPS）建立体外大鼠气道上皮细胞炎症损伤模型，采用ELISA检测气道上皮细胞分泌 TNF-α、IL-6和气道上皮细胞中 TLR4蛋白水平，RT-PCR检测气道上皮细胞TLR4 mRNA的水平。结果 LPS明显诱导大鼠气道上皮细胞分泌 TNF-α、IL-6，同时增强TLR4 mRNA和TLR4蛋白的表达（P＜0．01）。茶黄素可抑制LPS诱导TNF-α、IL-6的分泌和TLR4基因的表达（P＜0．05），并且与茶黄素药物浓度有关（P＜0．05）。结论在大鼠的气道上皮细胞培养中，茶黄素抗炎作用可能通过抑制 TLR4基因的表达、L PS/T L R4信号传导通路，从而减少炎性细胞因子的释放。
목적：연구다황소대원대배양적대서기도상피세포표체 TLR4급분비 TNF-α화 IL-6적영향。방법용지다당（LPS）건입체외대서기도상피세포염증손상모형，채용ELISA검측기도상피세포분비 TNF-α、IL-6화기도상피세포중 TLR4단백수평，RT-PCR검측기도상피세포TLR4 mRNA적수평。결과 LPS명현유도대서기도상피세포분비 TNF-α、IL-6，동시증강TLR4 mRNA화TLR4단백적표체（P＜0．01）。다황소가억제LPS유도TNF-α、IL-6적분비화TLR4기인적표체（P＜0．05），병차여다황소약물농도유관（P＜0．05）。결론재대서적기도상피세포배양중，다황소항염작용가능통과억제 TLR4기인적표체、L PS/T L R4신호전도통로，종이감소염성세포인자적석방。
Objective To study the influence of theaflavin on the expressions of TLR4 and release of TNF-αand IL-6 in primari-ly cultured rat airway epithelial cells .Methods Lipopolysacchride(LPS) was adopted to establish the in vitro inflammatory injury model of rat airway epithelial cell ,the TNF-αand IL-6 secreted by the airway epithelial cells and the TLR4 protein level in the air-way epithelial cells were detected with ELISA and the expressions of TLR4 mRNA was detected with RT-PCR .Results LPS sig-nificantly induced the airway epithelial cells to secrete TNF-αand IL-6 ,and enhanced the TLR4 mRNA and TLR4 protein expres-sion(P<0 .01) .Theaflavin could inhibit LPS induced TNF-αand IL-6 secretion and TLR4 gene expression(P<0 .05) ,which was related with the theaflavin concentration (P<0 .05) .Conclusion In rat airway epithelial cell culture ,the anti-inflammatory effect of theaflavin may be decrease the release of inflammatory cytokines via inhibiting TLR 4 gene expression and LPS/TLR4 signal trans-duction pathway .