重庆医学
重慶醫學
중경의학
CHONGQING MEDICAL JOURNAL
2014年
21期
2753-2755,2758
,共4页
杜雨柔%赵菊梅%张生军%李建%刘敏丽
杜雨柔%趙菊梅%張生軍%李建%劉敏麗
두우유%조국매%장생군%리건%류민려
乳腺肿瘤%细胞凋亡%细胞增殖%紫苏油
乳腺腫瘤%細胞凋亡%細胞增殖%紫囌油
유선종류%세포조망%세포증식%자소유
breast neoplasms%apoptosis%cell proliferation%basil oil
目的:探讨紫苏油对人乳腺癌系M C F7细胞的生长抑制和凋亡诱导作用,为开发紫苏油进行抗肿瘤提供理论基础。方法用不同稀释度的紫苏油处理人乳腺癌细胞系MCF7,做肿瘤细胞生长抑制的噻唑蓝(MTT)实验,Hoechst 33258与碘化丙啶(PI)染色荧光显微镜观察凋亡细胞的核形态改变,流式细胞术检测细胞凋亡率和凋亡峰。结果紫苏油对人乳腺癌细胞系MCF7的增殖有抑制作用,并有时间和浓度依赖性,而同类的大豆油没有此作用。用Hoechst 33258和PI染色观察到典型的凋亡细胞的核形态学改变。流式细胞术检测到细胞凋亡率随时间和紫苏油浓度的增加而增加。结论紫苏油具有抑制人乳腺癌M C F7细胞增殖和诱导细胞凋亡的作用,提示其有可能作为一种抗肿瘤药物应用于临床。
目的:探討紫囌油對人乳腺癌繫M C F7細胞的生長抑製和凋亡誘導作用,為開髮紫囌油進行抗腫瘤提供理論基礎。方法用不同稀釋度的紫囌油處理人乳腺癌細胞繫MCF7,做腫瘤細胞生長抑製的噻唑藍(MTT)實驗,Hoechst 33258與碘化丙啶(PI)染色熒光顯微鏡觀察凋亡細胞的覈形態改變,流式細胞術檢測細胞凋亡率和凋亡峰。結果紫囌油對人乳腺癌細胞繫MCF7的增殖有抑製作用,併有時間和濃度依賴性,而同類的大豆油沒有此作用。用Hoechst 33258和PI染色觀察到典型的凋亡細胞的覈形態學改變。流式細胞術檢測到細胞凋亡率隨時間和紫囌油濃度的增加而增加。結論紫囌油具有抑製人乳腺癌M C F7細胞增殖和誘導細胞凋亡的作用,提示其有可能作為一種抗腫瘤藥物應用于臨床。
목적:탐토자소유대인유선암계M C F7세포적생장억제화조망유도작용,위개발자소유진행항종류제공이론기출。방법용불동희석도적자소유처리인유선암세포계MCF7,주종류세포생장억제적새서람(MTT)실험,Hoechst 33258여전화병정(PI)염색형광현미경관찰조망세포적핵형태개변,류식세포술검측세포조망솔화조망봉。결과자소유대인유선암세포계MCF7적증식유억제작용,병유시간화농도의뢰성,이동류적대두유몰유차작용。용Hoechst 33258화PI염색관찰도전형적조망세포적핵형태학개변。류식세포술검측도세포조망솔수시간화자소유농도적증가이증가。결론자소유구유억제인유선암M C F7세포증식화유도세포조망적작용,제시기유가능작위일충항종류약물응용우림상。
Objective To investigate perilla oil on human breast cancer MCF7 cell growth inhibition and induction of apoptosis , and provide a theoretical basis for the development of perilla oil anti-tumor .Methods Breast cancer MCF7 cells were treated with different dilutions of perilla oil to do tumor cell growth inhibition MTT experiment ,to observe the changes in the nuclear morpholo-gy of apoptotic cells with Hoechst 33258 and PI staining and fluorescence microscopy ,and to detect rate of apoptosis and apoptotic peak with flow cytometry .Results Perilla oil inhibited the proliferation on human breast cancer cell line MCF7 with a time-and concentration-dependent manner .Typical apoptotic nuclear morphological changes could be observed with Hoechst 33258 and PI staining under a fluorescence microscope .Detected by flow cytometry ,apoptosis rate was increased with time and concentration . Conclusion Perilla oil can inhibit human breast cancer MCF7 cell proliferation and induce apoptosis ,suggesting that it may be used as an anticancer drug in clinical practice .