CAJ | 학술논문

目的：研究三七总皂苷（PNS）对糖尿病勃起功能障碍（ED）大鼠勃起功能的影响。方法90只雄性SD大鼠注射链脲佐菌素（STZ），构建糖尿病大鼠模型。利用阿朴吗啡诱导阴茎勃起，筛选出糖尿病ED大鼠。经过4周的 PNS处理后（分低、中、高剂量组），测定各组大鼠海绵体内压（ICP）值和平均动脉压（MAP）。硝酸还原酶法检测海绵体组织一氧化氮（NO）的水平， ELISA检测环磷酸鸟苷（cGM P）以及晚期糖基化总产物（AGEs）的水平。采用免疫组织化学法检测阴茎海绵体内皮细胞中内皮型一氧化氮合成酶（eNOS）的表达，末端脱氧核苷酸转移酶介导的dUTP缺口末端标记测定（TUNEL）法检测内皮细胞的凋亡水平。结果 PNS中、高剂量组的ICP值和ICP/MAP值较糖尿病对照组（未经PNS处理的糖尿病大鼠模型）明显升高（P＜0．05）。PNS中、高剂量组阴茎海绵体组织中的eNOS表达以及NO和cGMP水平较糖尿病对照组均有所增加（P＜0．05）。3个PNS治疗组阴茎海绵体的细胞凋亡率与糖尿病对照组相比明显降低（ P＜0．05）。结论 PNS通过调节 NO/cGM P信号通路和控制AGEs的积聚能够使阴茎海绵体内皮细胞功能恢复，可能有助于改善糖尿病ED大鼠的勃起功能。
목적：연구삼칠총조감（PNS）대당뇨병발기공능장애（ED）대서발기공능적영향。방법90지웅성SD대서주사련뇨좌균소（STZ），구건당뇨병대서모형。이용아박마배유도음경발기，사선출당뇨병ED대서。경과4주적 PNS처리후（분저、중、고제량조），측정각조대서해면체내압（ICP）치화평균동맥압（MAP）。초산환원매법검측해면체조직일양화담（NO）적수평， ELISA검측배린산조감（cGM P）이급만기당기화총산물（AGEs）적수평。채용면역조직화학법검측음경해면체내피세포중내피형일양화담합성매（eNOS）적표체，말단탈양핵감산전이매개도적dUTP결구말단표기측정（TUNEL）법검측내피세포적조망수평。결과 PNS중、고제량조적ICP치화ICP/MAP치교당뇨병대조조（미경PNS처리적당뇨병대서모형）명현승고（P＜0．05）。PNS중、고제량조음경해면체조직중적eNOS표체이급NO화cGMP수평교당뇨병대조조균유소증가（P＜0．05）。3개PNS치료조음경해면체적세포조망솔여당뇨병대조조상비명현강저（ P＜0．05）。결론 PNS통과조절 NO/cGM P신호통로화공제AGEs적적취능구사음경해면체내피세포공능회복，가능유조우개선당뇨병ED대서적발기공능。
Objective To investigate the effects of Panax notoginseng saponins(PNS) on penile erection in rats with diabetes-as-sociated erectile dysfunction (ED) .Methods Ninety male Sprague-Dawley rats were established diabetic rats by injecting streptozo-tocin(STZ) ,and observing erectile phenomenon by injecting apomorphine .After 4 weeks of PNS treatment (low-dose ,medium-dose , high-dose group) ,erectile function in each group was assessed by intracavernous pressure (ICP) and mean arterial pressure(MAP) measurement .The level of nitric oxide(NO)and cyclic guanosine monophosphate(cGMP)in cavernous tissue were detected .Immu-nohistochemical staining and TUNEL were performed for detecting endothelial nitric oxide synthase (eNOS) and apoptosis ,respec-tively .Results ICP and ICP/MAP ratio were significantly increased in medium-dose and high-dose PNS treated groups compared with the diabetic untreated group(P<0 .05) .Compared with the diabetic untreated group ,the expression of eNOS and the levels of NO and cGMP were increased in medium-dose and high-dose PNS treated groups(P<0 .05) .The apoptosis ratecorpus of caverno-sum in 3 PNS treatment groups significantly decreased than the diabetic untreated group (P<0 .05) .Conclusion PNS can recovery the endothelial cell function in corpus cavernosum by adjusting the NO/cGMP pathway and controlling the accumulation of AGEs , and may be used for improving in diabetic ED rats .