CAJ | 학술논문

目的：研究系统性红斑狼疮（SLE）患儿外周血滤泡辅助性T细胞相关细胞因子及受体表达水平。方法用ELISA检测22例SLE患儿和20例年龄、性别匹配的健康儿童（对照组）血清中的滤泡辅助性 T细胞相关细胞因子（CXCL13、IL-21）的水平，采用流式细胞仪检测滤泡辅助性 T 细胞相关受体（CXCR5、IL-21R）在两组儿童外周血CD19＋ B细胞中的表达。结果SLE患儿血清中CXCL13、IL-21的水平显著高于对照组（P＜0．01）。CXCR5在SLE患儿外周血B细胞上的表达显著低于对照组（P＜0．05），IL-21R在两组对象中差异无统计学意义（P＞0．05）。结论 SLE患儿血清中CXCL13、IL-21的水平升高提示滤泡辅助性T细胞在SLE患儿体内活性增高，对自身反应性B细胞可以产生过强的辅助信号，滤泡辅助性T细胞在SLE患儿的发病机制中起着重要的作用。
목적：연구계통성홍반랑창（SLE）환인외주혈려포보조성T세포상관세포인자급수체표체수평。방법용ELISA검측22례SLE환인화20례년령、성별필배적건강인동（대조조）혈청중적려포보조성 T세포상관세포인자（CXCL13、IL-21）적수평，채용류식세포의검측려포보조성 T 세포상관수체（CXCR5、IL-21R）재량조인동외주혈CD19＋ B세포중적표체。결과SLE환인혈청중CXCL13、IL-21적수평현저고우대조조（P＜0．01）。CXCR5재SLE환인외주혈B세포상적표체현저저우대조조（P＜0．05），IL-21R재량조대상중차이무통계학의의（P＞0．05）。결론 SLE환인혈청중CXCL13、IL-21적수평승고제시려포보조성T세포재SLE환인체내활성증고，대자신반응성B세포가이산생과강적보조신호，려포보조성T세포재SLE환인적발병궤제중기착중요적작용。
Objective To study the change of the plasma concentrations of follicular helper T lymphocytes (TFH cells) related cytokines and receptors in children with systemic lupus erythematosus (SLE) .Methods The plasma concentrations of TFH-cells-related cytokine IL-21 and CXCL13 ,and cell surface expression of TFH-related receptor CXCR5 and IL-21R on CD19+B cells in 22 SLE children and 20 sex-and age matched control subjects were measured by enzyme-linked immunosorbent assay and flow cytome-try ,respectively .Results Plasma CXCL13 and IL-21 concentrations were significantly higher in SLE children than controls (all P<0 .01) .Cell surface expression of CXCR5 on B cells was significantly lower in SLE children than controls (P<0 .05) .However ,the cell surface expression of IL-21R had no significant changes between SLE children and controls(P>0 .05) .Conclusion The elevat-ed production of CXCL13 and IL-21 may be associated with the function of TFH for the immunopathogenesis in SLE children .