CAJ | 학술논문

背景：作者前期制备了无细胞神经移植物，并将骨髓间充质干细胞种植其内，成功构建了组织工程人工神经。 　　目的：应用辣根过氧化物酶神经逆行示踪技术，评价无细胞神经移植物复合骨髓间充质干细胞构建的组织工程人工神经桥接大鼠坐骨神经缺损后对感觉神经元的保护作用。 　　方法：成年清洁级健康雄性SD大鼠，随机分成3组：实验组采用复合骨髓间充质干细胞的无细胞神经移植物桥接大鼠坐骨神经缺损；空白对照组采用无细胞神经移植物桥接大鼠坐骨神经缺损；自体神经对照组采用自体神经移植桥接大鼠坐骨神经缺损。移植后12周应用辣根过氧化物酶神经逆行示踪技术，对脊髓后根神经节感觉神经元的再生进行评价。 　　结果与结论：移植12周后根神经节感觉神经元再生评价结果提示，实验组与自体神经对照组相比差异无显著性意义，优于空白对照组；足底皮肤S-100免疫组织化学染色结果显示，各组皮下均见棕色阳性反应。提示无细胞神经移植物复合骨髓间充质干细胞构建组织工程人工神经修复大鼠坐骨神经缺损，对大鼠脊髓后根神经节感觉神经元具有保护作用，可以促进感觉功能的恢复。
배경：작자전기제비료무세포신경이식물，병장골수간충질간세포충식기내，성공구건료조직공정인공신경。 　　목적：응용랄근과양화물매신경역행시종기술，평개무세포신경이식물복합골수간충질간세포구건적조직공정인공신경교접대서좌골신경결손후대감각신경원적보호작용。 　　방법：성년청길급건강웅성SD대서，수궤분성3조：실험조채용복합골수간충질간세포적무세포신경이식물교접대서좌골신경결손；공백대조조채용무세포신경이식물교접대서좌골신경결손；자체신경대조조채용자체신경이식교접대서좌골신경결손。이식후12주응용랄근과양화물매신경역행시종기술，대척수후근신경절감각신경원적재생진행평개。 　　결과여결론：이식12주후근신경절감각신경원재생평개결과제시，실험조여자체신경대조조상비차이무현저성의의，우우공백대조조；족저피부S-100면역조직화학염색결과현시，각조피하균견종색양성반응。제시무세포신경이식물복합골수간충질간세포구건조직공정인공신경수복대서좌골신경결손，대대서척수후근신경절감각신경원구유보호작용，가이촉진감각공능적회복。
BACKGROUND:We have previously prepared acellular nerve graft and implanted bone marrow mesenchymal stem cells into the graft, to successful y construct tissue engineered artificial nerves. OBJECTIVE:Horseradish peroxidase nerve retrograde tracer technique was used to evaluate protective effects on sensory neurons fol owing sciatic nerve defect bridging with tissue engineered artificial nerves constructed by acellular nerve graft and bone marrow mesenchymal stem cells in rats. METHODS:Adult, clean, healthy, male Sprague-Dawley rats were randomly assigned to three group:(1) Experimental group:Rat sciatic nerve detect was bridged by acellular nerve graft combined with bone marrow mesenchymal stem cells;(2) Blank control group:Rat sciatic nerve defect was bridged by acellular nerve graft;(3) Autologous nerve control group:Rat sciatic nerve defect was bridged by autologous nerve transplantation. Regeneration of sensory neurons in the spinal dorsal root ganglia was assessed using horseradish peroxidase nerve retrograde tracer technique at 12 weeks fol owing surgery. RESULTS AND CONCLUSION:Sensory neuron regeneration in the spinal dorsal root ganglia at 12 weeks fol owing surgery was better in the experimental group compared with blank control group. No significant difference was detected between experimental group and autologous nerve control group. S-100 immunohistochemical staining in plantar skin showed brown positive reaction in each group. These findings indicate that tissue engineered artificial nerves constructed by acellular nerve graft and bone marrow mesenchymal stem cells have protective effects on sensory neurons in the spinal dorsal root ganglia, and can promote the recovery of sensory function and repair sciatic nerve defect in rats.