CAJ | 학술논문

背景：瘢痕疙瘩的发生是创伤修复的纤维化过程，多种纤维化相关细胞因子参与其发生发展。骨形态发生蛋白7、Gremlin与高迁移率族蛋白B1在其他器官纤维化中发挥重要作用，而在瘢痕疙瘩组织中的研究少见报道。 　　目的：分析骨形态发生蛋白7、Gremlin(骨形成蛋白拮抗剂家族成员)、血管内皮生长因子与高迁移率族蛋白B1在瘢痕疙瘩发病机制中的作用和意义。 　　方法：采用免疫组织化学方法及Wsetern Blot法检测骨形态发生蛋白7、Gremlin、血管内皮生长因子与高迁移率族蛋白B1在20例瘢痕疙瘩组织和20例正常皮肤组织中的分布及蛋白水平的表达，并对瘢痕疙瘩组织中骨形态发生蛋白7与Gremlin、血管内皮生长因子与高迁移率族蛋白B1的表达情况进行相关性分析。 　　结果与结论：与正常皮肤组织相比，Gremlin、血管内皮生长因子与高迁移率族蛋白B1在瘢痕疙瘩组织中表达呈显著性上调(P<0.01)，而骨形态发生蛋白7的表达呈显著性下调(P<0.01)，Gremlin与骨形态发生蛋白7在瘢痕疙瘩中的表达呈显著性负相关(r=-0.539，P <0.05)。血管内皮生长因子与高迁移率族蛋白B1在瘢痕疙瘩中的表达呈显著性正相关(r=0.560，P <0.05)。说明Gremlin的过度表达和骨形态发生蛋白7的表达下调，以及高迁移率族蛋白B1与血管内皮生长因子的高表达可能共同参与了瘢痕疙瘩的纤维化病理过程。
배경：반흔흘탑적발생시창상수복적섬유화과정，다충섬유화상관세포인자삼여기발생발전。골형태발생단백7、Gremlin여고천이솔족단백B1재기타기관섬유화중발휘중요작용，이재반흔흘탑조직중적연구소견보도。 　　목적：분석골형태발생단백7、Gremlin(골형성단백길항제가족성원)、혈관내피생장인자여고천이솔족단백B1재반흔흘탑발병궤제중적작용화의의。 　　방법：채용면역조직화학방법급Wsetern Blot법검측골형태발생단백7、Gremlin、혈관내피생장인자여고천이솔족단백B1재20례반흔흘탑조직화20례정상피부조직중적분포급단백수평적표체，병대반흔흘탑조직중골형태발생단백7여Gremlin、혈관내피생장인자여고천이솔족단백B1적표체정황진행상관성분석。 　　결과여결론：여정상피부조직상비，Gremlin、혈관내피생장인자여고천이솔족단백B1재반흔흘탑조직중표체정현저성상조(P<0.01)，이골형태발생단백7적표체정현저성하조(P<0.01)，Gremlin여골형태발생단백7재반흔흘탑중적표체정현저성부상관(r=-0.539，P <0.05)。혈관내피생장인자여고천이솔족단백B1재반흔흘탑중적표체정현저성정상관(r=0.560，P <0.05)。설명Gremlin적과도표체화골형태발생단백7적표체하조，이급고천이솔족단백B1여혈관내피생장인자적고표체가능공동삼여료반흔흘탑적섬유화병리과정。
BACKGROUND:The development of keloid is a progress of fibrosis in wound healing, and involves various fibrosis-related cytokines. Bone morphogenetic protein 7 (BMP7), Gremlin and high mobility group box-1 (HMGB1) play an important role in fibrosis of many organs, but their role in keloid tissue has rarely been reported. OBJECTIVE:To investigate the role of BMP7, Gremlin, vascular endothelial growth factor (VEGF) and HMGB1 in the development of keloid. METHODS:The protein levels and distribution of BMP7, Gremlin, VEGF and HMGB1 in 20 cases of keloid and 20 cases of normal skin were detected by immunohistochemistry and western blot analysis, respectively. And the correlations among expression levels of BMP7, Gremlin, VEGF and HMGB1 in keloid were analyzed. RESULTS AND CONCLUSION:In keloid tissue, the expression levels of Gremlin, VEGF and HMGB1 were significantly higher than that in normal skin (P<0.01), while the expression levels of BMP7 were significantly lower (P<0.01). The levels of Gremlin were negatively correlated with the levels of BMP7 (r=-0.539, P<0.05). And the levels of VEGF were positively correlated with the levels of HMGB1 (r=0.56, P<0.05). The overexpression of Gremlin and decreased expression of BMP7, as wel as the increased expression of HMGB1 and VEGF, may contribute to the pathogenesis of fibrosis in the development of keloid.