中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2014年
30期
4868-4872
,共5页
高喜翔%张建%陈兵%谷涌泉%李建新%张淑文%叶霖%冯增国
高喜翔%張建%陳兵%穀湧泉%李建新%張淑文%葉霖%馮增國
고희상%장건%진병%곡용천%리건신%장숙문%협림%풍증국
生物材料%共聚物%ε-己内酯%L-丙交酯%生物可降解材料%低密度聚乙烯%组织工程人工血管%药物控释%国家自然科学基金
生物材料%共聚物%ε-己內酯%L-丙交酯%生物可降解材料%低密度聚乙烯%組織工程人工血管%藥物控釋%國傢自然科學基金
생물재료%공취물%ε-기내지%L-병교지%생물가강해재료%저밀도취을희%조직공정인공혈관%약물공석%국가자연과학기금
polyethylene%biodegradation,environmental%polymers
背景:可降解高分子材料在植入体内后其降解过程就开始启动,如何能控制可降解材料降解过程的启动,做到定时、定位启动,国内外鲜有研究。目的:研究超声波对高分子材料降解过程启动的控制。方法:将ε-己内酯/L-丙交酯共聚物制成样本的可降解内核,在其表面通过4种方法涂覆低密度聚乙烯疏水涂层:①先将内核表面黏附一薄层CaCl 2粉末,而后将聚乙烯涂覆于其表面。②先将聚乙烯涂覆于内核表面,静置粗化3 h。③将内核表面黏附一薄层CaCl 2粉末,而后将聚乙烯涂覆于其表面,静置粗化3h。④直接将聚乙烯涂覆于内核表面。将4种样本包埋于带皮猪肉内进行体外超声波轰击实验。结果与结论:方法1、4制备的样本,在轰击前其疏水层能够保护内核材料,其浸泡液在631 nm处无吸收峰值;轰击后由于疏水层破损,甲苯胺蓝染料被释放出来,造成溶液颜色改变及631 nm处吸收峰值明显升高。方法2、3制备的样本,在轰击前其疏水层未能起到保护作用,其浸泡液在631 nm处出现吸收峰值。电镜扫描显示4组样本在超声波作用下发生了明显表面容貌变化。表明采用低密度聚乙烯制作的疏水层可以保护内核ε-己内酯/L-丙交酯共聚物材料,以体外超声波作为诱发因素,可以控制可降解内核降解过程的启动。
揹景:可降解高分子材料在植入體內後其降解過程就開始啟動,如何能控製可降解材料降解過程的啟動,做到定時、定位啟動,國內外鮮有研究。目的:研究超聲波對高分子材料降解過程啟動的控製。方法:將ε-己內酯/L-丙交酯共聚物製成樣本的可降解內覈,在其錶麵通過4種方法塗覆低密度聚乙烯疏水塗層:①先將內覈錶麵黏附一薄層CaCl 2粉末,而後將聚乙烯塗覆于其錶麵。②先將聚乙烯塗覆于內覈錶麵,靜置粗化3 h。③將內覈錶麵黏附一薄層CaCl 2粉末,而後將聚乙烯塗覆于其錶麵,靜置粗化3h。④直接將聚乙烯塗覆于內覈錶麵。將4種樣本包埋于帶皮豬肉內進行體外超聲波轟擊實驗。結果與結論:方法1、4製備的樣本,在轟擊前其疏水層能夠保護內覈材料,其浸泡液在631 nm處無吸收峰值;轟擊後由于疏水層破損,甲苯胺藍染料被釋放齣來,造成溶液顏色改變及631 nm處吸收峰值明顯升高。方法2、3製備的樣本,在轟擊前其疏水層未能起到保護作用,其浸泡液在631 nm處齣現吸收峰值。電鏡掃描顯示4組樣本在超聲波作用下髮生瞭明顯錶麵容貌變化。錶明採用低密度聚乙烯製作的疏水層可以保護內覈ε-己內酯/L-丙交酯共聚物材料,以體外超聲波作為誘髮因素,可以控製可降解內覈降解過程的啟動。
배경:가강해고분자재료재식입체내후기강해과정취개시계동,여하능공제가강해재료강해과정적계동,주도정시、정위계동,국내외선유연구。목적:연구초성파대고분자재료강해과정계동적공제。방법:장ε-기내지/L-병교지공취물제성양본적가강해내핵,재기표면통과4충방법도복저밀도취을희소수도층:①선장내핵표면점부일박층CaCl 2분말,이후장취을희도복우기표면。②선장취을희도복우내핵표면,정치조화3 h。③장내핵표면점부일박층CaCl 2분말,이후장취을희도복우기표면,정치조화3h。④직접장취을희도복우내핵표면。장4충양본포매우대피저육내진행체외초성파굉격실험。결과여결론:방법1、4제비적양본,재굉격전기소수층능구보호내핵재료,기침포액재631 nm처무흡수봉치;굉격후유우소수층파손,갑분알람염료피석방출래,조성용액안색개변급631 nm처흡수봉치명현승고。방법2、3제비적양본,재굉격전기소수층미능기도보호작용,기침포액재631 nm처출현흡수봉치。전경소묘현시4조양본재초성파작용하발생료명현표면용모변화。표명채용저밀도취을희제작적소수층가이보호내핵ε-기내지/L-병교지공취물재료,이체외초성파작위유발인소,가이공제가강해내핵강해과정적계동。
BACKGROUND:Degradable polymer materials initiate the degradation process immediately after implantation. How to regulate the degradation of these materials is rarely reported at present. OBJECTIVE:To study the effect of ultrasonic wave on control ing the degradation of polymer materials. METHODS:The sample is made ofε-caprolactone/L-lactide copolymer, and its core was coated with low density polyethylene on the surface with the fol owing four different methods. (1) The core surface was firstly covered with CaCl 2 powder, and then coated with polyethylene. (2) The core was firstly coated with polyethylene and coarsened for 3 hours. (3) The core surface was firstly covered with CaCl 2 powder, and then coated with polyethylene, and coarsened for 3 hours. (4) The core was directly coated with polyethylene. The four kinds of specimens obtained were embedded in pork for ultrasonic bombardment experiment in vitro. RESULTS AND CONCLUSION:In the specimens prepared with methods 1 and 4, the lyophobic layer could protect core materials before ultrasonic treatment, and no absorption peak was found at 631 nm. After ultrasonic treatment, the lyophobic layer was destroyed, toluidine blue dye was released, leading to change the color of immersion solution and increase the absorption peak at 631 nm. In the specimens prepared with methods 2 and 3,the lyophobic layer cannot exhibit the protection effects, the absorption peak was found at 631 nm. Under electron microscope, the appearance of the specimens in four groups was changed obviously. It is feasible to control the starting of the degradation by coating the degradable copolymer with LDPE and using ultrasonic as a trigger.