CAJ | 학술논문

背景：实验证明胶原蛋白底物具有刺激成软骨的作用，但关于不同类型胶原蛋白刺激成软骨作用的能力仍存在争议。目的：观察Ⅰ、Ⅱ型胶原蛋白对体外培养人软骨细胞生物学特性的影响。方法：将P3代人软骨细胞分别加入普通培养板、Ⅰ型胶原蛋白包被培养板、Ⅱ型胶原蛋白包被培养板继续培养。培养10 d内，MTT法绘制细胞生长曲线；培养28 d后，采用ELISA法、聚合酶链反应、二甲基亚甲基蓝比色等方法检测3种培养板中软骨细胞分泌Ⅰ胶原蛋白、Ⅱ型胶原蛋白及糖胺多糖的量。结果与结论：Ⅱ型胶原蛋白包被培养板中软骨细胞数量最多，增殖速度为Ⅰ型胶原蛋白包被培养板的2倍、普通培养板的5倍。Ⅱ型胶原蛋白包被培养板中软骨细胞分泌Ⅰ型胶原蛋白最少，与普通培养板板检测结果差异有显著性意义(P<0.01)，与Ⅰ型胶原蛋白包被培养板检测结果差异无显著性意义；Ⅱ型胶原蛋白包被培养板中软骨细胞分泌Ⅱ型胶原蛋白、糖胺多糖最多，与其他两种培养板检测结果差异有显著性意义（P<0.01）。表明胶原蛋白包被培养板培养软骨细胞优于普通培养板，其中Ⅱ型胶原蛋白包被培养板在培养软骨细胞时更能维持细胞形态，延长去分化现象出现的时间，更利于细胞再分化。
배경：실험증명효원단백저물구유자격성연골적작용，단관우불동류형효원단백자격성연골작용적능력잉존재쟁의。목적：관찰Ⅰ、Ⅱ형효원단백대체외배양인연골세포생물학특성적영향。방법：장P3대인연골세포분별가입보통배양판、Ⅰ형효원단백포피배양판、Ⅱ형효원단백포피배양판계속배양。배양10 d내，MTT법회제세포생장곡선；배양28 d후，채용ELISA법、취합매련반응、이갑기아갑기람비색등방법검측3충배양판중연골세포분비Ⅰ효원단백、Ⅱ형효원단백급당알다당적량。결과여결론：Ⅱ형효원단백포피배양판중연골세포수량최다，증식속도위Ⅰ형효원단백포피배양판적2배、보통배양판적5배。Ⅱ형효원단백포피배양판중연골세포분비Ⅰ형효원단백최소，여보통배양판판검측결과차이유현저성의의(P<0.01)，여Ⅰ형효원단백포피배양판검측결과차이무현저성의의；Ⅱ형효원단백포피배양판중연골세포분비Ⅱ형효원단백、당알다당최다，여기타량충배양판검측결과차이유현저성의의（P<0.01）。표명효원단백포피배양판배양연골세포우우보통배양판，기중Ⅱ형효원단백포피배양판재배양연골세포시경능유지세포형태，연장거분화현상출현적시간，경리우세포재분화。
BACKGROUND:Experiments have shown that the col agen substrate has the capability of stimulating cartilage generation, but the stimulating role of different types of col agen substrates remains controversial. OBJECTIVE:To investigate the effect of type I and type II col agen on the biological characteristics of human chondrocytes cultured in vitro. METHODS:Human chondrocytes at passage were cultured onto the ordinary culture plates (ordinary plate), type I col agen-coated culture plates (type I plate), and type II col agen-coated culture plates (type II plate). cellgrowth curves were determined by MTT method after cells were cultured for 10 days. By ELISA, PCR, and 1,9-dimethyl methyleneblue technology, type I and type II col agen and glycosaminoglycan contents were quantitatively detected in cartilage cells 28 days after culture. RESULTS AND CONCLUSION:The number of cartilage cells was the highest in type II plate, which was twice of that in type I plate and five times of that in ordinary plate. Cartilage cells in type II plate secreted the least amount of type I col agen, which showed significant differences compared with the ordinary plate (P<0.01) and had no statistical y significant difference with type I plate (P>0.01). Cartilage cells in type II plate secreted the most amount of type II col agen and glycosaminoglycan, showing significant differences compared with the other two plates (P<0.01). The cartilage cells cultured in col agen plates are better than that cultured in ordinary culture plate, type II col agen culture plate is better than type I col agen culture plate in maintaining cellshape, extending the dedifferentiation pattern, and promoting celldifferentiation.