CAJ | 학술논문

背景：含铍合金口腔修复体在口腔湿润的环境中合金易被腐蚀、溶解而释放出铍离子，铍离子对牙周病主要致病菌--牙龈卟啉单胞菌菌毛基因型是否有影响？目的：检测铍离子对牙龈卟啉单胞菌菌毛基因型的影响。方法：将复苏后的牙龈卟啉单胞菌置于含铍离子浓度为10×10-6，5×10-6，1.25×10-648 h，采用PCR扩增、测序的方法从分子水平检测铍离子对牙龈卟啉单胞菌菌毛基因型的影响。结果与结论：在实验条件下培养后的牙龈卟啉单胞菌fimA基因出现突变现象，表现为：当铍离子浓度为5×10时，DNA 217、和257位点处出现G/A套峰，与其他组测得的碱基G不同，表明此处有可疑的碱基变化，部分碱基由G转变为A；所有浓度都发现DNA 101位点处插入由7个A碱基组成的碱基(段)，目前研究尚未发现突变的发生与铍离子浓度存在直接相关关系。铍离子可影响牙龈卟啉单胞菌fimA基因发生变化，可能引起其阅读框架的移位及编码的蛋白合成异常，进而引起牙龈卟啉单胞菌黏附能力及致病力的改变。的人工唾液中厌氧培养-6
배경：함피합금구강수복체재구강습윤적배경중합금역피부식、용해이석방출피리자，피리자대아주병주요치병균--아간계람단포균균모기인형시부유영향？목적：검측피리자대아간계람단포균균모기인형적영향。방법：장복소후적아간계람단포균치우함피리자농도위10×10-6，5×10-6，1.25×10-648 h，채용PCR확증、측서적방법종분자수평검측피리자대아간계람단포균균모기인형적영향。결과여결론：재실험조건하배양후적아간계람단포균fimA기인출현돌변현상，표현위：당피리자농도위5×10시，DNA 217、화257위점처출현G/A투봉，여기타조측득적감기G불동，표명차처유가의적감기변화，부분감기유G전변위A；소유농도도발현DNA 101위점처삽입유7개A감기조성적감기(단)，목전연구상미발현돌변적발생여피리자농도존재직접상관관계。피리자가영향아간계람단포균fimA기인발생변화，가능인기기열독광가적이위급편마적단백합성이상，진이인기아간계람단포균점부능력급치병력적개변。적인공타액중염양배양-6
BACKGROUND:In oral warm and moisture circumstance, al oy which contains Be is easily to be eroded to release Be2+. But there is stil no research focusing on beryl ium influence on genotype of Porphyromonas gingivalis fimbriae gene cluster. OBJECTIVE:To investigate Be 2+effect on genotype of Porphyromonas gingivalis fimbriae gene cluster. METHODS:The revived Porphyromonas gingivalis was resuscitated for 48 hours in the anaerobic culture medium with different concentration of Be 2+(10×10-6, 5×10-6, 1.25×10-6). Through PCR amplification and sequencing, we investigated the effects of Be2+RESULTS AND CONCLUSION:(1) When Be on genotypes of Porphyromonas gingivalis fimbriae gene cluster. 2+concentration was 5×10-6, we found the peak of 217 and 257 sites on DNA sequence expressing G/A overlap peak, different from G single peak of the other groups, suggesting the suspicious bases changes, part of the single base G mutated into A. (2) On al concentrations, we found a base group composed of seven A bases was inserted into the 101 site of DNA sequence. Up to now, there is no direct contacts of the mutations occurring to Be2+concentration. Changes of gene may lead to the shifting of the reading frame, the abnormal synthesis of proteins, the change of Porphyromonas gingivalis fimA gene toxicity, and lastly the unbalance of the micro-ecological environment.