CAJ | 학술논문

背景：前期实验中曾发现纤维的取向可以引导平滑肌细胞的取向生长，因此，设想通过制备取向排列的电纺丝纤维支架，以引导食管平滑肌细胞的有序生长，从而有利于维持肌细胞的形貌及生物功能。目的：以可降解聚己内酯、明胶、丝素蛋白为基材，采用自制的电纺丝系统制备无规和有序的纳米级多孔纤维。方法：将聚己内酯与丝素蛋白以4∶1质量比混合，通过调整溶液浓度、电压、喷射速度等参数，采用自制的电纺丝系统制备聚己内酯/丝素蛋白电纺丝纤维。将聚己内酯与明胶分别以2∶1、1∶1、1∶2质量比混合，在金属平板接收器下，通过调整溶液浓度、电压、喷射速度等参数，采用自制的电纺丝系统制备聚己内酯/明胶无规电纺丝纤维；同时改用滚轴接收装置，通过调整滚轴转数、电压、喷射速度等参数，制备聚己内酯/明胶有序电纺丝纤维。结果与结论：在溶液质量浓度为0.08 g/mL、纺丝液流速1.6 mL/h和电压22.5 kV的条件下，制得了均匀、无串珠、纤维直径为(535.9±126.7) nm的聚己内酯/丝素蛋白多孔纳米纤维膜。在溶液质量浓度为0.10 g/mL、纺丝液流速0.8 mL/h和电压22.5 kV的条件下，制得了无明显串珠、纤维直径为(257.9±117.8) nm的聚己内酯/明胶多孔纳米纤维膜；并且在1∶2质量比时更易成纤维，纤维尺寸更均匀。在滚轴转速3000 r/min。溶液流速0.8 mL/h。电压15 kV的条件下，制得的聚己内酯/明胶有序电纺丝纤维排序更理想，纤维也更均匀。
배경：전기실험중증발현섬유적취향가이인도평활기세포적취향생장，인차，설상통과제비취향배렬적전방사섬유지가，이인도식관평활기세포적유서생장，종이유리우유지기세포적형모급생물공능。목적：이가강해취기내지、명효、사소단백위기재，채용자제적전방사계통제비무규화유서적납미급다공섬유。방법：장취기내지여사소단백이4∶1질량비혼합，통과조정용액농도、전압、분사속도등삼수，채용자제적전방사계통제비취기내지/사소단백전방사섬유。장취기내지여명효분별이2∶1、1∶1、1∶2질량비혼합，재금속평판접수기하，통과조정용액농도、전압、분사속도등삼수，채용자제적전방사계통제비취기내지/명효무규전방사섬유；동시개용곤축접수장치，통과조정곤축전수、전압、분사속도등삼수，제비취기내지/명효유서전방사섬유。결과여결론：재용액질량농도위0.08 g/mL、방사액류속1.6 mL/h화전압22.5 kV적조건하，제득료균균、무천주、섬유직경위(535.9±126.7) nm적취기내지/사소단백다공납미섬유막。재용액질량농도위0.10 g/mL、방사액류속0.8 mL/h화전압22.5 kV적조건하，제득료무명현천주、섬유직경위(257.9±117.8) nm적취기내지/명효다공납미섬유막；병차재1∶2질량비시경역성섬유，섬유척촌경균균。재곤축전속3000 r/min。용액류속0.8 mL/h。전압15 kV적조건하，제득적취기내지/명효유서전방사섬유배서경이상，섬유야경균균。
BACKGROUND:We have found that oriented fibers can guide the alignment of smooth muscle cells in our previous experiments. Thus, we designed the experiment to prepare wel aligned polymeric fibers using electrospinning technology, aiming at guiding the growth of esophageal smooth muscle cells to maintain cellmorphology and biological function. OBJECTIVE:Using electrospinning technology, to fabricate isotropic and directed nano-fibrous scaffolds made of polycaprolacton, gelatin and silk fibroin. METHODS:Polycaprolacton/silk fibroin fibers at a ratio of 4:1 were prepared with proper parameters, including solution concentration, voltage and injection speed, under the self-made spinning system. The polycaprolacton/gelatin sheets with mass ratio of 2:1, 1:1 and 1:2, respectively, were also fabricated under suitable process parameters. Using the rol er col ector instead of the metal plate, polycaprolacton/gelatin nano-fibrous scaffold with good alignment of fibers was manufactured. RESULTS AND CONCLUSION:The isotropic polycaprolacton/silk fibroin scaffold with fiber diameter of (535.9±126.7) nm was prepared under conditions of solution concentration (0.08 g/mL), injection speed (1.6 mL/h) and voltage (22.5 kV), and these fibers were uniform with no beads. The isotropic polycaprolacton/gelatin scaffold with fiber diameter of (257.9±117.8) nm was prepared under conditions of solution concentration (0.10 g/mL), injection speed (0.8 mL/h) and voltage (22.5 kV). Using the rol er col ector instead of the previous metal plate, polycaprolacton/gelatin (w:w, 1:2) nano-fibrous scaffold with good alignment of fibers was manufactured. The process parameters were 3 000 r/min of rol ing speed, 0.8 mL/h of injection speed and 15 kV of voltage.