中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2014年
30期
4764-4770
,共7页
生物材料%软骨生物材料%富血小板血浆%软骨细胞%软骨组织工程%组织再生%生长因子
生物材料%軟骨生物材料%富血小闆血漿%軟骨細胞%軟骨組織工程%組織再生%生長因子
생물재료%연골생물재료%부혈소판혈장%연골세포%연골조직공정%조직재생%생장인자
platelet-rich plasma%chondrocytes%tissue engineering%cartilage
背景：富血小板血浆中含有大量的生长因子，因此其在骨再生、创伤愈合等方面得到了较多的应用，然而其在组织工程软骨的研究报道较少。目的：观察富血小板血浆对软骨细胞增殖和分化的影响，以及富血小板血浆复合软骨细胞构建组织工程化软骨的可行性。方法：检测兔全血、富血小板血浆及激活富血小板血浆中转化生长因子β、胰岛素样生长因子1、血小板源性生长因子BB及表皮生长因子的浓度。将兔软骨细胞在分别含10%，15%，20%，30%富血小板血浆的DMEM培养液中培养7 d，CCK-8法检测细胞增殖，QT-PCR检测细胞内Ⅱ型胶原、蛋白聚糖、Sox-9的表达。在兔皮下注射自体富血小板血浆与软骨细胞复合物，6周后取材进行组织学检测。结果与结论：富血小板血浆中各生长因子浓度高于全血(P<0.05)，激活富血小板血浆中各生长因子浓度高于富血小板血浆(P<0.05)。不同浓度富血小板血浆均能促进软骨细胞的增殖，且20%浓度内呈浓度依赖性。20%浓度组促Ⅱ型胶原表达的能力强于其他浓度组(P<0.05)，15%浓度组促Sox-9和蛋白聚糖表达的能力强于其他浓度组(P <0.05)。富血小板血浆-软骨细胞复合物移植后，新生组织呈软骨样并有明显的软骨陷窝，细胞外富含软骨样基质，表明其作为可注射性支架用于软骨组织工程。
揹景：富血小闆血漿中含有大量的生長因子，因此其在骨再生、創傷愈閤等方麵得到瞭較多的應用，然而其在組織工程軟骨的研究報道較少。目的：觀察富血小闆血漿對軟骨細胞增殖和分化的影響，以及富血小闆血漿複閤軟骨細胞構建組織工程化軟骨的可行性。方法：檢測兔全血、富血小闆血漿及激活富血小闆血漿中轉化生長因子β、胰島素樣生長因子1、血小闆源性生長因子BB及錶皮生長因子的濃度。將兔軟骨細胞在分彆含10%，15%，20%，30%富血小闆血漿的DMEM培養液中培養7 d，CCK-8法檢測細胞增殖，QT-PCR檢測細胞內Ⅱ型膠原、蛋白聚糖、Sox-9的錶達。在兔皮下註射自體富血小闆血漿與軟骨細胞複閤物，6週後取材進行組織學檢測。結果與結論：富血小闆血漿中各生長因子濃度高于全血(P<0.05)，激活富血小闆血漿中各生長因子濃度高于富血小闆血漿(P<0.05)。不同濃度富血小闆血漿均能促進軟骨細胞的增殖，且20%濃度內呈濃度依賴性。20%濃度組促Ⅱ型膠原錶達的能力彊于其他濃度組(P<0.05)，15%濃度組促Sox-9和蛋白聚糖錶達的能力彊于其他濃度組(P <0.05)。富血小闆血漿-軟骨細胞複閤物移植後，新生組織呈軟骨樣併有明顯的軟骨陷窩，細胞外富含軟骨樣基質，錶明其作為可註射性支架用于軟骨組織工程。
배경：부혈소판혈장중함유대량적생장인자，인차기재골재생、창상유합등방면득도료교다적응용，연이기재조직공정연골적연구보도교소。목적：관찰부혈소판혈장대연골세포증식화분화적영향，이급부혈소판혈장복합연골세포구건조직공정화연골적가행성。방법：검측토전혈、부혈소판혈장급격활부혈소판혈장중전화생장인자β、이도소양생장인자1、혈소판원성생장인자BB급표피생장인자적농도。장토연골세포재분별함10%，15%，20%，30%부혈소판혈장적DMEM배양액중배양7 d，CCK-8법검측세포증식，QT-PCR검측세포내Ⅱ형효원、단백취당、Sox-9적표체。재토피하주사자체부혈소판혈장여연골세포복합물，6주후취재진행조직학검측。결과여결론：부혈소판혈장중각생장인자농도고우전혈(P<0.05)，격활부혈소판혈장중각생장인자농도고우부혈소판혈장(P<0.05)。불동농도부혈소판혈장균능촉진연골세포적증식，차20%농도내정농도의뢰성。20%농도조촉Ⅱ형효원표체적능력강우기타농도조(P<0.05)，15%농도조촉Sox-9화단백취당표체적능력강우기타농도조(P <0.05)。부혈소판혈장-연골세포복합물이식후，신생조직정연골양병유명현적연골함와，세포외부함연골양기질，표명기작위가주사성지가용우연골조직공정。
BACKGROUND:Owing to containing large amounts of growth factors, platelet-rich plasma has been widely used in bone regeneration, wound healing, and so on, while few studies have been reported on cartilage tissue engineering. OBJECTIVE:To explore the effects of platelet-rich plasma on the proliferation and differentiation of chondrocytes, and the feasibility of constructing tissue-engineered cartilage by the combination of chondrocytes and platelet-rich plasma. METHODS:We detected the concentrations of transforming growth factor-β, insulin-like growth factor-1, platelet-derived growth factor and epidermal growth factor BB in the whole blood, platelet-rich plasma, and activated platelet-rich plasma. The rabbit articular chondrocytes were cultured in 10%, 15%, 20%, 30%platelet-rich plasma for 7 days, then the cellproliferation was tested by cellcounting kit-8, and cartilage-related genes (col agen type II, Aggrecan, Sox-9) were determined using QT-PCR. The chondrocytes/platelet-rich plasma composite was implanted subcutaneously into the rabbits, and the samples were harvested after 6 weeks of transplantation for histological examination. RESULTS AND CONCLUSION:The levels of different growth factors in the platelet-rich plasma were higher than those in the whole blood (P<0.05), but lower than those in the activated platelet-rich plasma (P<0.05). Platelet-rich plasma at different concentrations promoted the proliferation of chondrocytes. When the concentration of platelet-rich plasma was no more than 20%, the proliferation of chondrocytes showed a concentration-dependent manner. The 20%platelet-rich plasma showed the best effects to promote the expression of col agen type II (P<0.05), while the 15%platelet-rich plasma could maximize the expression of Sox-9 and Aggrecan (P<0.05). After transplantation of chondrocytes/platelet-rich plasma composite, cartilage-like tissue formed with cartilage lacuna-like structures, and rich extracellular matrix was found, which indicates platelet-rich plasma can be used as an injective scaffold in cartilage tissue engineering.
