热带林业
熱帶林業
열대임업
TROPICAL FORESTRY
2014年
2期
15-17
,共3页
愈伤组织%外植体%生长调节剂%诱导%增殖
愈傷組織%外植體%生長調節劑%誘導%增殖
유상조직%외식체%생장조절제%유도%증식
Callus%Explant%Phytohormone%Induction%Reproduction
该文以琼岛杨为研究对象，研究外植体、消毒方法，以及植物激素对琼岛杨愈伤组织的诱导、增殖和分化的影响。研究结果表明：琼岛杨以叶片作为外植体，0.1%的升汞消毒6min.，无菌水冲洗5次比较理想。激素配比6-BA 0.5mg/L+2-4D 1.0mg/L 能更好地诱导和促进愈伤组织的生长，增值率达到了80%，诱导的愈伤组织呈现翠绿色，颗粒饱满；激素配比6-BA 2.0mg/L+ IBA 0.5mg/L 有利于愈伤组织分化出带绒毛的芽点。
該文以瓊島楊為研究對象，研究外植體、消毒方法，以及植物激素對瓊島楊愈傷組織的誘導、增殖和分化的影響。研究結果錶明：瓊島楊以葉片作為外植體，0.1%的升汞消毒6min.，無菌水遲洗5次比較理想。激素配比6-BA 0.5mg/L+2-4D 1.0mg/L 能更好地誘導和促進愈傷組織的生長，增值率達到瞭80%，誘導的愈傷組織呈現翠綠色，顆粒飽滿；激素配比6-BA 2.0mg/L+ IBA 0.5mg/L 有利于愈傷組織分化齣帶絨毛的芽點。
해문이경도양위연구대상，연구외식체、소독방법，이급식물격소대경도양유상조직적유도、증식화분화적영향。연구결과표명：경도양이협편작위외식체，0.1%적승홍소독6min.，무균수충세5차비교이상。격소배비6-BA 0.5mg/L+2-4D 1.0mg/L 능경호지유도화촉진유상조직적생장，증치솔체도료80%，유도적유상조직정현취록색，과립포만；격소배비6-BA 2.0mg/L+ IBA 0.5mg/L 유리우유상조직분화출대융모적아점。
The article studies the effect of the explant, disinfection method, and phytohormone on the callus in-duction, reproduction and differentiation of Populus qiongdaoensis T. Hong et P. Luo. The results showed that it was a better way using the leaf as explant sterilized by 0.1% (w/v) aqueous mercuric chloride solution for 6 min with sterile water washing for 5 times. The combination of 6-BA 0.5 mg/L and 2-4 d 1.0 mg/L would be better to promote the growth of callus, and the induction rate of reproduction reached 80%. The induction of callus presented emerald and starlatine. The ratio of phytohormone supplemented 6 - BA 2.0 mg/L with IBA 0.5 mg/L was conducive to the callus differentiate into bud points with fluff.
