海洋湖沼通报
海洋湖沼通報
해양호소통보
TRANSACTIONS OF OCEANOLOGY AND LIMNOLOGY
2014年
2期
141-146
,共6页
极端嗜盐古菌%筛选%鉴定%C50类胡萝卜素
極耑嗜鹽古菌%篩選%鑒定%C50類鬍蘿蔔素
겁단기염고균%사선%감정%C50류호라복소
extremely halophilic archaea%isolation%identification%C50 carotenoid
从日晒盐场结晶池中筛选到1株产C50类胡萝卜素的红色极端嗜盐古菌。该菌株为革兰氏阴性菌,短棒状,最适生长盐度为250,最适生长pH 为7。表型鉴定方法结合16S rDNA序列分析判定,该菌属于极端嗜盐古菌盐红菌属 Halorubrum,命名为 Halorubrum sp.Sp1(16S rRNA Genbank 登录号为KF697239)。根据紫外-可见光扫描特征光谱,确定该菌株主要色素为 C50类胡萝卜素。pH 8时单位细胞色素积累量最大,在盐度150~300范围内随盐度升高,单位细胞色素积累量逐渐降低。
從日曬鹽場結晶池中篩選到1株產C50類鬍蘿蔔素的紅色極耑嗜鹽古菌。該菌株為革蘭氏陰性菌,短棒狀,最適生長鹽度為250,最適生長pH 為7。錶型鑒定方法結閤16S rDNA序列分析判定,該菌屬于極耑嗜鹽古菌鹽紅菌屬 Halorubrum,命名為 Halorubrum sp.Sp1(16S rRNA Genbank 登錄號為KF697239)。根據紫外-可見光掃描特徵光譜,確定該菌株主要色素為 C50類鬍蘿蔔素。pH 8時單位細胞色素積纍量最大,在鹽度150~300範圍內隨鹽度升高,單位細胞色素積纍量逐漸降低。
종일쇄염장결정지중사선도1주산C50류호라복소적홍색겁단기염고균。해균주위혁란씨음성균,단봉상,최괄생장염도위250,최괄생장pH 위7。표형감정방법결합16S rDNA서렬분석판정,해균속우겁단기염고균염홍균속 Halorubrum,명명위 Halorubrum sp.Sp1(16S rRNA Genbank 등록호위KF697239)。근거자외-가견광소묘특정광보,학정해균주주요색소위 C50류호라복소。pH 8시단위세포색소적루량최대,재염도150~300범위내수염도승고,단위세포색소적루량축점강저。
An extremely halophilic C50 carotenoid-producing red archaeon was isolated from the crystalli-zer ponds in solar saltworks.The isolated strain is Gram-negative and short rod.The optimum salinity and pH for growth is 250 and 7,respectively.Phenotypic and molecular analyses of this strain indicated that it belonged to extremely halophilic archaea genus Halorubrum and named Halorubrum Sp1 (16S rRNA Genbank registration number KF697239).UV-visible scanning spectrum showed that C50 carote-noid was the major pigments presented in this strain.Pigment accumulation was maximizing at pH 8. In the salinity range of 150~300,increasing salinity resulted in declined pigment accumulation.
