CAJ | 학술논문

采用 RAPD 分子标记技术，分析了金钗石斛、铁皮石斛和齿瓣石斛三种石斛属植物的种间关系。10个引物产生的113条DNA扩增片段中，106条（93.81%）具有多态性，利用113个RAPD标记，计算遗传距离，利用非加权组平均法建立聚类图。结果表明，RAPD标记技术较好地从分子水平揭示金钗石斛、铁皮石斛和齿瓣石斛三种石斛属植物的遗传背景、亲缘关系，并为后期在DNA水平上对药用石斛的开发利用提供资料。
채용 RAPD 분자표기기술，분석료금차석곡、철피석곡화치판석곡삼충석곡속식물적충간관계。10개인물산생적113조DNA확증편단중，106조（93.81%）구유다태성，이용113개RAPD표기，계산유전거리，이용비가권조평균법건립취류도。결과표명，RAPD표기기술교호지종분자수평게시금차석곡、철피석곡화치판석곡삼충석곡속식물적유전배경、친연관계，병위후기재DNA수평상대약용석곡적개발이용제공자료。
Three species of Dendrobium, D. nobile, D. officinale and D. devonianum were analyzed using random amplified polymorphic DNA (RAPD) markers. A total of 113 bands were amplified by 10 RAPD primers, among which 106 (93.81%) bands were polymorphic. The data of 113 RAPD bands were used to generate genetic distances and to construct a dendrogram by unweighted pair group method analysis (UPGMA). The results showed that the RAPD approach was efficient in studying genetic background and relationships among these 3 species and could be used to provide abundant molecular data for the medicinal development and utilization of Dendrobium.