CAJ | 학술논문

蔷薇藻Rhodella reticulata是属于红藻门的一种海洋单细胞微藻，其生长过程中产生藻胆蛋白、胞外多糖等生物活性物质。蔷薇藻经过破碎，通过硫酸铵沉淀和DEAE Sepharose FF柱层析后，可以分离出较纯的藻蓝蛋白。本文从pH、温度、光照、食品添加剂、金属离子等方面对蔷薇藻藻蓝蛋白稳定性作了较全面的研究：藻蓝蛋白在pH为7时最稳定；低温有利于保持藻蓝蛋白的活性；相对于光照，在避光条件下，藻蓝蛋白有较高的稳定性；适当浓度的蔗糖和葡萄糖都有利于藻蓝蛋白的保存；金属离子影响藻蓝蛋白的稳定性。
장미조Rhodella reticulata시속우홍조문적일충해양단세포미조，기생장과정중산생조담단백、포외다당등생물활성물질。장미조경과파쇄，통과류산안침정화DEAE Sepharose FF주층석후，가이분리출교순적조람단백。본문종pH、온도、광조、식품첨가제、금속리자등방면대장미조조람단백은정성작료교전면적연구：조람단백재pH위7시최은정；저온유리우보지조람단백적활성；상대우광조，재피광조건하，조람단백유교고적은정성；괄당농도적자당화포도당도유리우조람단백적보존；금속리자영향조람단백적은정성。
The unicellular red alga, Rhodella reticulata, can synthesize some bioactive substances such as phycobiliprotein and exopolysaccharides. After extraction by fractional precipitation with ammonium sulfate, phycocyanion (PC) was purified by ion-exchange chromatography on DEAE-Sepharose Fast Flow column. In this paper, effects of various parameters such as pH, temperature, illumination, food additives and metal ions on the stability of PC of Rhodella reticulate were studied. PC has the most stable activity in pH 7;Lower temperature is in favour of the activity of PC, so do appropriate amount of sucrose and glucose; It had higher stability away from light;Metal ions affect the stability of PC.