CAJ | 학술논문

实验室条件下研究了光照强度及周期性波动对凡纳滨对虾(Litopenaeus vannamei)Na+-K+-ATP酶活力的影响。实验设60、600、1500、3000和6000 lx5个恒定光照组，以及600~60 lx、1500~60 lx、3000~60 lx和6000~60 lx 4个光照强度周期性突变组。结果发现，凡纳滨对虾肌肉中 Na+-K+-ATP酶活力具有明显的昼夜节律，但不同光照模式下其规律性有所差异；较大幅度的光照强度突变导致对虾 Na+-K+-ATP 酶活力先迅速上升，然后缓慢下降，最后缓慢回升；不同光照模式下，对虾 Na+-K+-ATP酶活力均值有所差异，其中1500 lx组对虾Na+-K+-ATP酶活力值均值最低，这可能是对虾处于1种生理平衡状态，离子运输和渗透压调节保持在较低水平；较大幅度的光照强度突变处理组中对虾的 Na+-K+-ATP酶活力均值要显著小于其对应的恒定光照处理组，其主要原因可能与机体的代谢及相关酶的功效不同有关。
실험실조건하연구료광조강도급주기성파동대범납빈대하(Litopenaeus vannamei)Na+-K+-ATP매활력적영향。실험설60、600、1500、3000화6000 lx5개항정광조조，이급600~60 lx、1500~60 lx、3000~60 lx화6000~60 lx 4개광조강도주기성돌변조。결과발현，범납빈대하기육중 Na+-K+-ATP매활력구유명현적주야절률，단불동광조모식하기규률성유소차이；교대폭도적광조강도돌변도치대하 Na+-K+-ATP 매활력선신속상승，연후완만하강，최후완만회승；불동광조모식하，대하 Na+-K+-ATP매활력균치유소차이，기중1500 lx조대하Na+-K+-ATP매활력치균치최저，저가능시대하처우1충생리평형상태，리자운수화삼투압조절보지재교저수평；교대폭도적광조강도돌변처리조중대하적 Na+-K+-ATP매활력균치요현저소우기대응적항정광조처리조，기주요원인가능여궤체적대사급상관매적공효불동유관。
Four periodic fluctuation light intensity treatments (600~60 lx,1500~60 lx,3000~60 lx and 6000~60 lx)and five constant light intensity treatments (60 lx,600 lx,1500 lx,3000 lx,6000 lx) were arranged to investigate the effect of periodic abrupt change in light intensities and constant light intensities on Na+-K+-ATPase.After assay and analyze the Na+-K+-ATPase activity,it can be found that:the fluctuation of Na+-K+-ATPase had certain rhythmicity,but the rhythmicity in different light intensity regimes were difference.The Na+-K+-ATPase activity increased rapidly when light intensity abrupt changed with a more light amplitude,then it decreased gradually;after hours the Na+-K+-AT-Pase activity increased gradually again.The average activities of Na+-K+-ATPase were different in dif-ferent light intensity regimes,the average activities of Na+-K+-ATPase of shrimps in treatment 1500 lx were lowest,the reason for those results maybe that shrimps in light treatment 1500 lx were in a physi-ological equilibrium,and the osmoregulation and ion transportation were in a lower level.The average activities of Na+-K+-ATPase of shrimps in treatment 3000~60 lx and 6000~60 lx were significantly lower than those in the corresponding constant treatment 60 lx,3000 lx and 6000 lx,the reason may be that the metabolism and effectiveness of the enzyme were different.