海洋湖沼通报
海洋湖沼通報
해양호소통보
TRANSACTIONS OF OCEANOLOGY AND LIMNOLOGY
2014年
2期
23-30
,共8页
梁英%刘春强%田传远%孙明辉
樑英%劉春彊%田傳遠%孫明輝
량영%류춘강%전전원%손명휘
海洋微藻%氮源%磷源%营养盐输入方式%群落演替
海洋微藻%氮源%燐源%營養鹽輸入方式%群落縯替
해양미조%담원%린원%영양염수입방식%군락연체
marine microalgae%nitrogen sources%phosphorous sources%nutrient input modes%commu-nity succession
在不同的氮源(硝酸钠、氯化铵、尿素)、不同的磷源(磷酸二氢钠、甘油磷酸钠、三磷酸腺苷二钠)和不同的营养盐输入方式(一次性输入、每2d输入、每5d 输入)下,对三角褐指藻、小新月菱形藻、杜氏盐藻、青岛大扁藻、米氏凯伦藻和前沟藻进行混合培养实验,研究营养盐形态及输入方式对6种海洋微藻群落演替的影响。实验结果表明,以硝酸钠和氯化铵为氮源时,硅藻为优势种,分别占总细胞密度的56.0%和58.4%,而以尿素为氮源时,绿藻有一定的优势,占总细胞密度的56.5%;以磷酸二氢钠和甘油磷酸钠为磷源时硅藻具有竞争优势,分别占总细胞密度的56.0%和59.6%,但以三磷酸腺苷二钠为磷源时,绿藻为优势种,占总细胞密度的80.0%;不同的营养盐输入方式下,硅藻都能保持一定的竞争优势,一次性输入、每2d输入、每5d输入方式下,硅藻分别占总细胞密度的63.2%、85.6%和60.4%,其中每2d输入方式下,硅藻竞争优势最大。在所有的处理组中,甲藻都受到严重的抑制,只能占有很小的比例。
在不同的氮源(硝痠鈉、氯化銨、尿素)、不同的燐源(燐痠二氫鈉、甘油燐痠鈉、三燐痠腺苷二鈉)和不同的營養鹽輸入方式(一次性輸入、每2d輸入、每5d 輸入)下,對三角褐指藻、小新月蔆形藻、杜氏鹽藻、青島大扁藻、米氏凱倫藻和前溝藻進行混閤培養實驗,研究營養鹽形態及輸入方式對6種海洋微藻群落縯替的影響。實驗結果錶明,以硝痠鈉和氯化銨為氮源時,硅藻為優勢種,分彆佔總細胞密度的56.0%和58.4%,而以尿素為氮源時,綠藻有一定的優勢,佔總細胞密度的56.5%;以燐痠二氫鈉和甘油燐痠鈉為燐源時硅藻具有競爭優勢,分彆佔總細胞密度的56.0%和59.6%,但以三燐痠腺苷二鈉為燐源時,綠藻為優勢種,佔總細胞密度的80.0%;不同的營養鹽輸入方式下,硅藻都能保持一定的競爭優勢,一次性輸入、每2d輸入、每5d輸入方式下,硅藻分彆佔總細胞密度的63.2%、85.6%和60.4%,其中每2d輸入方式下,硅藻競爭優勢最大。在所有的處理組中,甲藻都受到嚴重的抑製,隻能佔有很小的比例。
재불동적담원(초산납、록화안、뇨소)、불동적린원(린산이경납、감유린산납、삼린산선감이납)화불동적영양염수입방식(일차성수입、매2d수입、매5d 수입)하,대삼각갈지조、소신월릉형조、두씨염조、청도대편조、미씨개륜조화전구조진행혼합배양실험,연구영양염형태급수입방식대6충해양미조군락연체적영향。실험결과표명,이초산납화록화안위담원시,규조위우세충,분별점총세포밀도적56.0%화58.4%,이이뇨소위담원시,록조유일정적우세,점총세포밀도적56.5%;이린산이경납화감유린산납위린원시규조구유경쟁우세,분별점총세포밀도적56.0%화59.6%,단이삼린산선감이납위린원시,록조위우세충,점총세포밀도적80.0%;불동적영양염수입방식하,규조도능보지일정적경쟁우세,일차성수입、매2d수입、매5d수입방식하,규조분별점총세포밀도적63.2%、85.6%화60.4%,기중매2d수입방식하,규조경쟁우세최대。재소유적처리조중,갑조도수도엄중적억제,지능점유흔소적비례。
In this paper,six marine microalgal species(Phaeodactylum tricornutum,Nitzschia closterium f.minutissima,Dunaliella salina,Platymonas helgolandica var.tsingtaoensi,Karenia mikimotoi and Amphidinium carterae)were mixed cultured under different nitrogen sources (NaNO3,NH4Cl,CO (NH2)2),different phosphorous sources (NaH2PO4,glycerophosphate,adenosine triphosphate diso-dium)and different nutrient input modes (input one time,input every 2 days,input every 5 days)in order to study the effects of nutrient sources and input modes on the community succession of six mi-croalgal species.The results showed that when NaNO3 and NH4Cl were nitrogen sources,diatoms were the dominant algae and accounted for 56.0% and 58.4% of the total cell densities,respectively, while green algae had competitive advantage when urea was nitrogen source and accounted for 56.5% of the total cell density.When NaH2PO4 and glycerophosphate were phosphorus sources,diatoms were the competitive species and accounted for 56.0% and 59.6% of the total cell densities,respectively, while green algae were dominants when adenosine triphosphate disodium was phosphorus source,ac-counting for 80.0% of the total cell density.The diatoms were all dominant in different nutrient input modes and accounted for 63.2%,85.6% and 60.4% of the total cell densities,respectively,when the nutrients were input one time,input every 2 days and input every 5 days,with the highest competitive-ness occurred in every 2 days input mode.Dinoflagellates were inhibited and only occupied small per-centages of the total cell densities in all treatments.