中华脑科疾病与康复杂志（电子版）
中華腦科疾病與康複雜誌（電子版）
중화뇌과질병여강복잡지（전자판）
CHINESE JOURNAL OF BRAIN DI8SEASES AND REHABILITATIN(ELECTRONIC EDITION)
2014年
3期
21-25
,共5页
李芸%柳弥%吴碧华%王冠
李蕓%柳瀰%吳碧華%王冠
리예%류미%오벽화%왕관
糖尿病,2型%胰高血糖素样肽1%紧密连接相关蛋白5
糖尿病,2型%胰高血糖素樣肽1%緊密連接相關蛋白5
당뇨병,2형%이고혈당소양태1%긴밀련접상관단백5
Diabetes mellitus,type 2%Glucagon like peptide 1%Claudin-5
目的：研究糖尿病大鼠脑微血管内皮细胞胰高血糖素样肽-1受体（ GLP-1R）与紧密连接相关蛋白5（Claudin-5）的表达变化，初步探讨GLP-1在糖尿病大鼠脑微血管病变中的可能机制。方法（1）8周龄雄性SD大鼠60只，随机分为高脂组（模型组，30只）与普通饲料组（对照组，30只），分别予以高脂饲料和普通饲料喂养8周。（2）8周后高脂组予以腹腔注射链脲佐菌素（STZ）30 g/kg，建立2型糖尿病（T2DM）大鼠模型。（3）成模4周后测定大鼠体重及空腹血糖变化。（4）处死各组大鼠，免疫组化和Western blot检测各组大鼠脑微血管内皮细胞上GLP-1R及Claudin-5蛋白表达情况。采用SPSS 13.0统计软件，计量资料用均数±标准差（ x珋±s）表示，数据经正态检验后采用完全随机设计t 检验。结果（1）对照组大鼠体重由基础值（163.00±10.10） g 增加至（331.10±17.40）g；模型组大鼠体重由基础值（165.00±12.10）g增加至（421.30±18.82）g，两组组内前后比较差异有统计学意义（t值分别为-100.935、-104.124，均P＜0.05）。（2）模型组大鼠空腹血糖由基础值（5.370±0.552）mmol/L升高为4周时的（22.100±3.069）mmol/L ，显著高于相应时间点对照组（t＝29.190，P＜0.05）。（3）成模8周后模型组大鼠海马皮质微血管内皮细胞上， GLP1-R 表达弱于对照组（ t＝-31.787，P＜0.05），且Claudin-5表达呈弱阳性，对照组大鼠Claudin-5呈强阳性表达（ t＝
目的：研究糖尿病大鼠腦微血管內皮細胞胰高血糖素樣肽-1受體（ GLP-1R）與緊密連接相關蛋白5（Claudin-5）的錶達變化，初步探討GLP-1在糖尿病大鼠腦微血管病變中的可能機製。方法（1）8週齡雄性SD大鼠60隻，隨機分為高脂組（模型組，30隻）與普通飼料組（對照組，30隻），分彆予以高脂飼料和普通飼料餵養8週。（2）8週後高脂組予以腹腔註射鏈脲佐菌素（STZ）30 g/kg，建立2型糖尿病（T2DM）大鼠模型。（3）成模4週後測定大鼠體重及空腹血糖變化。（4）處死各組大鼠，免疫組化和Western blot檢測各組大鼠腦微血管內皮細胞上GLP-1R及Claudin-5蛋白錶達情況。採用SPSS 13.0統計軟件，計量資料用均數±標準差（ x珋±s）錶示，數據經正態檢驗後採用完全隨機設計t 檢驗。結果（1）對照組大鼠體重由基礎值（163.00±10.10） g 增加至（331.10±17.40）g；模型組大鼠體重由基礎值（165.00±12.10）g增加至（421.30±18.82）g，兩組組內前後比較差異有統計學意義（t值分彆為-100.935、-104.124，均P＜0.05）。（2）模型組大鼠空腹血糖由基礎值（5.370±0.552）mmol/L升高為4週時的（22.100±3.069）mmol/L ，顯著高于相應時間點對照組（t＝29.190，P＜0.05）。（3）成模8週後模型組大鼠海馬皮質微血管內皮細胞上， GLP1-R 錶達弱于對照組（ t＝-31.787，P＜0.05），且Claudin-5錶達呈弱暘性，對照組大鼠Claudin-5呈彊暘性錶達（ t＝
목적：연구당뇨병대서뇌미혈관내피세포이고혈당소양태-1수체（ GLP-1R）여긴밀련접상관단백5（Claudin-5）적표체변화，초보탐토GLP-1재당뇨병대서뇌미혈관병변중적가능궤제。방법（1）8주령웅성SD대서60지，수궤분위고지조（모형조，30지）여보통사료조（대조조，30지），분별여이고지사료화보통사료위양8주。（2）8주후고지조여이복강주사련뇨좌균소（STZ）30 g/kg，건립2형당뇨병（T2DM）대서모형。（3）성모4주후측정대서체중급공복혈당변화。（4）처사각조대서，면역조화화Western blot검측각조대서뇌미혈관내피세포상GLP-1R급Claudin-5단백표체정황。채용SPSS 13.0통계연건，계량자료용균수±표준차（ x류±s）표시，수거경정태검험후채용완전수궤설계t 검험。결과（1）대조조대서체중유기출치（163.00±10.10） g 증가지（331.10±17.40）g；모형조대서체중유기출치（165.00±12.10）g증가지（421.30±18.82）g，량조조내전후비교차이유통계학의의（t치분별위-100.935、-104.124，균P＜0.05）。（2）모형조대서공복혈당유기출치（5.370±0.552）mmol/L승고위4주시적（22.100±3.069）mmol/L ，현저고우상응시간점대조조（t＝29.190，P＜0.05）。（3）성모8주후모형조대서해마피질미혈관내피세포상， GLP1-R 표체약우대조조（ t＝-31.787，P＜0.05），차Claudin-5표체정약양성，대조조대서Claudin-5정강양성표체（ t＝
Objective To research the expression of glucagon like peptide-1 receptor ( GLP-1R) and Claudin-5 on diabetic rat brain microvascular endothelial cells , to discuss the possible mechanism of GLP-1 in diabetic rat brain microvascular lesions .Mtehods ( 1 )60 male SD rats ( 8 weeks old ) were randomly divided into high fat group (30 rats) and conventional feeding group (30 rats).The rats were given high fat diet or normal diet 4 weeks.(2) After fed high fat diet 8 weeks, given the model group by intraperitoneal injection of streptozotocin (STZ) 30 mg/kg to establish type 2 diabetes (T2DM) model.(3) The change of each group of rats body weight and fasting blood glucose were measured after 4 weeks.(4) Immunohistochemistry and Western blot were adopted to detect GLP-1R and Claudin-5 expression on rat brain micro-vessel endothelial cell .Using SPSS 13.0 statistical software , the measurement data was represented by x±s, after the normal examination , data using completely randomized design test . Rseults (1) The general feeding group rats weight increases from (163.00 ±10.10 )g to (331.10 ±17.40 ) g;The T2DM model group increases from (165.00 ±12.10 ) g to (421.30 ±18.82 ) g,there were significant differences between two groups (t=-100.935,-104.124,all P<0.05).(2)The model group rats fasting glucose of(5.370 ±0.552)mmol/L increased to(22.100 ±3.069)mmol/L,was significantly higher than that in the corresponding time points,the normal control group(t=29.190,P<0.05).(3)The model group rats hippocampus cortex microvascular endothelial cells ,GLP1-R was weakly than that of the normal control group ( t=-31.787, P <0.05 ) , the expression of Claudin-5 was weakly positive , normal control rats in the Claudin-5 group showed strong positive expression ( t=-24.288 ,P<0.05 ) .Conclusion High glucose can also cut the the expression of Claudin-5 and GLP-1R on microvascular endothelial cells .
