中国临床医学
中國臨床醫學
중국림상의학
CLINICAL MEDICAL JOURNAL OF CHINA
2014年
3期
265-268
,共4页
都勇%揭志军%何燕超%钱凌
都勇%揭誌軍%何燕超%錢凌
도용%게지군%하연초%전릉
肺癌%着色性干皮病基因D%X线交叉互补修复基因1
肺癌%著色性榦皮病基因D%X線交扠互補脩複基因1
폐암%착색성간피병기인D%X선교차호보수복기인1
Lung cancer%Xeroderma pigmentosum D%X-ray repair cross complementing gene 1
目的：探讨着色性干皮病基因D（Xeroderma pigmentosum gene D ，XPD）和X线交叉互补修复基因1（X-ray repair cross complementing gene 1，XRCC1）的多态性与肺癌之间的相关性。方法：采用聚合酶链反应-限制性片段长度多态性（polyme-rase chain reaction-restriction fragment length polymorphism ，PCR-RFLP）技术，对150例肺癌患者（肺癌组）以及150例健康志愿者（健康对照组）的XPD-751、XRCC1-399两个多态位点进行基因型分析。结果：肺癌组及健康对照组XPD-751的基因型频率及突变等位基因频率差异有统计学意义（P分别为0．046、0．003）；肺癌组及健康对照组的XRCC1-399基因型频率及突变等位基因频率则差异无统计学意义（P分别为0．395、0．105）。非小细胞肺癌组和小细胞肺癌组XPD-751的突变等位基因频率差异有统计学意义（P＝0．012），鳞癌组、腺癌组及其他类型非小细胞肺癌组XPD-751的突变等位基因频率差异亦有统计学意义（P＝0．023）。吸烟和XPD-751多态性两个危险因素共同存在时，罹患肺癌的相对危险度为2．812（95％ CI：0．956～6．415），P＝0．336。结论：XPD-751基因多态性与肺癌的发生相关，尤其与非小细胞肺癌中的鳞癌关系密切；而XRCC1-399基因多态性则与肺癌的发生无关；吸烟和XPD-751多态性在肺癌的发生中无交互作用。
目的：探討著色性榦皮病基因D（Xeroderma pigmentosum gene D ，XPD）和X線交扠互補脩複基因1（X-ray repair cross complementing gene 1，XRCC1）的多態性與肺癌之間的相關性。方法：採用聚閤酶鏈反應-限製性片段長度多態性（polyme-rase chain reaction-restriction fragment length polymorphism ，PCR-RFLP）技術，對150例肺癌患者（肺癌組）以及150例健康誌願者（健康對照組）的XPD-751、XRCC1-399兩箇多態位點進行基因型分析。結果：肺癌組及健康對照組XPD-751的基因型頻率及突變等位基因頻率差異有統計學意義（P分彆為0．046、0．003）；肺癌組及健康對照組的XRCC1-399基因型頻率及突變等位基因頻率則差異無統計學意義（P分彆為0．395、0．105）。非小細胞肺癌組和小細胞肺癌組XPD-751的突變等位基因頻率差異有統計學意義（P＝0．012），鱗癌組、腺癌組及其他類型非小細胞肺癌組XPD-751的突變等位基因頻率差異亦有統計學意義（P＝0．023）。吸煙和XPD-751多態性兩箇危險因素共同存在時，罹患肺癌的相對危險度為2．812（95％ CI：0．956～6．415），P＝0．336。結論：XPD-751基因多態性與肺癌的髮生相關，尤其與非小細胞肺癌中的鱗癌關繫密切；而XRCC1-399基因多態性則與肺癌的髮生無關；吸煙和XPD-751多態性在肺癌的髮生中無交互作用。
목적：탐토착색성간피병기인D（Xeroderma pigmentosum gene D ，XPD）화X선교차호보수복기인1（X-ray repair cross complementing gene 1，XRCC1）적다태성여폐암지간적상관성。방법：채용취합매련반응-한제성편단장도다태성（polyme-rase chain reaction-restriction fragment length polymorphism ，PCR-RFLP）기술，대150례폐암환자（폐암조）이급150례건강지원자（건강대조조）적XPD-751、XRCC1-399량개다태위점진행기인형분석。결과：폐암조급건강대조조XPD-751적기인형빈솔급돌변등위기인빈솔차이유통계학의의（P분별위0．046、0．003）；폐암조급건강대조조적XRCC1-399기인형빈솔급돌변등위기인빈솔칙차이무통계학의의（P분별위0．395、0．105）。비소세포폐암조화소세포폐암조XPD-751적돌변등위기인빈솔차이유통계학의의（P＝0．012），린암조、선암조급기타류형비소세포폐암조XPD-751적돌변등위기인빈솔차이역유통계학의의（P＝0．023）。흡연화XPD-751다태성량개위험인소공동존재시，리환폐암적상대위험도위2．812（95％ CI：0．956～6．415），P＝0．336。결론：XPD-751기인다태성여폐암적발생상관，우기여비소세포폐암중적린암관계밀절；이XRCC1-399기인다태성칙여폐암적발생무관；흡연화XPD-751다태성재폐암적발생중무교호작용。
Objective:To investigate the correlation between lung cancer and the polymorphism of xeroderma pigmentosum gene D(XPD) and X-ray repair cross complementing gene 1 (XRCC1) .Methods :The polymorphisms at loci XPD-751 and XRCC1-399 in 150 patients with lung cancer (lung cancer group) and 150 healthy volunteers (control group) were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) .Results:There were significant differences in XPD-751 genotype frequency and mutant allele frequency between lung cancer group and control group (P= 0 .046 , P=0 .003) ,while XRCC1-399 loci had no significant difference(P=0 .395 ,P=0 .105) between the two groups .There were signif-icant differences in XPD-751 mutant allele frequency between patients with non-small cell lung cancer and patients with small cell lung cancer(P=0 .012) ,as well as among squamous cell carcinoma ,adenocarcinoma and other types of non-small cell lung cancer(P=0 .023) .When smoking and XPD-751 polymorphisms coexisted as risk factors ,the relative risk to lung cancer was 2 .812(95% CI:0 .956~6 .415) ,P=0 .336 .Conclusions :XPD-751 gene polymorphism was correlated with the occurrence of lung cancer ,especially with squamous cell lung carcinoma ,while XRCC1-399 gene polymorphism was not correlated with lung cancer .There is no interaction between smoking and the polymorphism of XPD-751 in lung cancer .
