CAJ | 학술논문

目的：研究microRNAs （miRNAs）在脂多糖（LPS）诱导的结肠癌细胞 HT-29炎性反应模型中的表达。方法：用400 ng/mL的LPS处理HT-29细胞48 h ，构建HT-29细胞炎性反应模型（LPS组），将未经LPS处理的 HT-29细胞作为对照组；抽提细胞总RNA ，检测HT-29细胞中白介素-6（IL-6）、C反应蛋白（CRP）的表达水平；用颈环结构引物反转录miRNAs ，应用实时荧光定量PCR测定其表达水平。结果：LPS组中IL-6和CRP的表达较对照组显著升高（P＜0．01），表明建模成功。LPS组miR-22、miR-26、miR-214的表达水平较对照组低，而 miR-23的表达水平较对照组高（ P＜0．05）；两组 miR-24、miR-30、miR-181的表达水平差异无统计学意义。结论：miRNAs可能参与LPS诱导的HT-29结肠癌细胞的炎性反应过程。
목적：연구microRNAs （miRNAs）재지다당（LPS）유도적결장암세포 HT-29염성반응모형중적표체。방법：용400 ng/mL적LPS처리HT-29세포48 h ，구건HT-29세포염성반응모형（LPS조），장미경LPS처리적 HT-29세포작위대조조；추제세포총RNA ，검측HT-29세포중백개소-6（IL-6）、C반응단백（CRP）적표체수평；용경배결구인물반전록miRNAs ，응용실시형광정량PCR측정기표체수평。결과：LPS조중IL-6화CRP적표체교대조조현저승고（P＜0．01），표명건모성공。LPS조miR-22、miR-26、miR-214적표체수평교대조조저，이 miR-23적표체수평교대조조고（ P＜0．05）；량조 miR-24、miR-30、miR-181적표체수평차이무통계학의의。결론：miRNAs가능삼여LPS유도적HT-29결장암세포적염성반응과정。
Objective:To study the expression of microRNAs (miRNAs) in colon adenocarcinoma cell line HT-29 with lipopo-lysaccharide-induced(LPS-induced) inflammation .Methods :HT-29 cells were treated with 400 ng/mL LPS for 48 h to establish inflammatory HT-29 cell model (LPS group) ,and HT-29 cells without LPS treatment were viewed as the control group .Total RNA from the two groups was isolated with Trizol reagent ,and IL-6 and CRP were examined .The expression of miRNAs was determined by Real-time PCR .Results:Compared with the control group ,the mRNA levels of IL-6 and CRP were up-regulated in LPS group .The expression levels of miR-22 ,miR-26 ,miR-214 in LPS group were lower than those in the control group , while miR-23 level was higher than that in the control group .The levels of miR-24 ,miR-30 and miR-181 were not statistically different between the two groups .Conclusions :MiRNAs may play important roles in LPS-induced inflammation of adenocarci-noma cells .