CAJ | 학술논문

目的：研究干扰素α对肝癌细胞中转录因子Sp1（specificity protein 1）的表达和活性的影响。方法：采用蛋白免疫印迹法分析干扰素α对肝癌细胞株M HCC97H中Sp1的表达及磷酸化水平的影响。采用凝胶电泳迁移率变动分析法检测干扰素α对S p1活性的影响。结果：干扰素α可显著下调S p1的表达和磷酸化水平，以对磷酸化水平的下调更为明显；干扰素α可显著抑制S p1的活性。结论：S p1可能作为干扰素α抗肝癌的临床疗效的预测指标。
목적：연구간우소α대간암세포중전록인자Sp1（specificity protein 1）적표체화활성적영향。방법：채용단백면역인적법분석간우소α대간암세포주M HCC97H중Sp1적표체급린산화수평적영향。채용응효전영천이솔변동분석법검측간우소α대S p1활성적영향。결과：간우소α가현저하조S p1적표체화린산화수평，이대린산화수평적하조경위명현；간우소α가현저억제S p1적활성。결론：S p1가능작위간우소α항간암적림상료효적예측지표。
Objective:To evaluate the effect of interferon-αon the expression and activity of transcription factor specificity pro-tein 1(Sp1) in hepatocarcinoma cells .Methods :The effect of interferon-αon the expression and phosphorylation level of Sp1 in hepatocarcinoma cell line M HCC97H were detected by Western blotting .The effect of interferon-α on the activity of Sp1 was analyzed by electrophoretic mobility shift assay (EMSA) .Results :Interferon-α significantly reduced the expression and phos-phorylation level of Sp1 ,and the reducement on phosphorylation was more distinct .Interferon-α also inhibited the activity of Sp1 significantly .Conclusions :Interferon-αinhibits the activity of Sp1 by down regulating the expression and phosphorylation level of Sp1 .Sp1 may be an optional predictor of clinical efficacy of interferon-αon hepatocarcinoma .