中国临床医学
中國臨床醫學
중국림상의학
CLINICAL MEDICAL JOURNAL OF CHINA
2014年
3期
237-238
,共2页
施国明%蔡加彬%俞静娴%柯爱武%董兆如%张弛%张鹏飞%邱双健%孙惠川%周俭%樊嘉
施國明%蔡加彬%俞靜嫻%柯愛武%董兆如%張弛%張鵬飛%邱雙健%孫惠川%週儉%樊嘉
시국명%채가빈%유정한%가애무%동조여%장이%장붕비%구쌍건%손혜천%주검%번가
小鼠模型%肝大部分切除术%肝再生
小鼠模型%肝大部分切除術%肝再生
소서모형%간대부분절제술%간재생
Mouse model%Partial hepatectomy%Liver regeneration
目的：探讨小鼠肝脏大部分切除后再生模型的改良制作方法。方法：将8～10周龄雄性C57BL/6小鼠腹腔麻醉后，用带针6-0 proline缝线环绕肝左外叶、肝中叶共同肝蒂，距肝蒂0．2 cm穿肝右中叶表面固定，结扎肝蒂，于结扎线远侧0．2 cm处切除小鼠肝脏左外叶、肝中叶（左、右）。观察小鼠术后成活率以及术后3d、7d、14d小鼠肝脏质量。结果：成功建立小鼠肝脏大部分切除模型，该模型切除小鼠肝左外叶、肝中叶（左、右），切除肝质量占肝总质量的66．7％，术后小鼠存活率100％。术后小鼠剩肝代偿性增生，术后第3天恢复至原肝总质量的58．2％，第7天恢复至原肝总质量的78．0％，术后第14天恢复至原肝总质量的93．2％。结论：本研究建立的改进型小鼠肝脏大部分切除后再生模型，具有简便、可操作性强、成功率高等优点。
目的：探討小鼠肝髒大部分切除後再生模型的改良製作方法。方法：將8～10週齡雄性C57BL/6小鼠腹腔痳醉後，用帶針6-0 proline縫線環繞肝左外葉、肝中葉共同肝蒂，距肝蒂0．2 cm穿肝右中葉錶麵固定，結扎肝蒂，于結扎線遠側0．2 cm處切除小鼠肝髒左外葉、肝中葉（左、右）。觀察小鼠術後成活率以及術後3d、7d、14d小鼠肝髒質量。結果：成功建立小鼠肝髒大部分切除模型，該模型切除小鼠肝左外葉、肝中葉（左、右），切除肝質量佔肝總質量的66．7％，術後小鼠存活率100％。術後小鼠剩肝代償性增生，術後第3天恢複至原肝總質量的58．2％，第7天恢複至原肝總質量的78．0％，術後第14天恢複至原肝總質量的93．2％。結論：本研究建立的改進型小鼠肝髒大部分切除後再生模型，具有簡便、可操作性彊、成功率高等優點。
목적：탐토소서간장대부분절제후재생모형적개량제작방법。방법：장8～10주령웅성C57BL/6소서복강마취후，용대침6-0 proline봉선배요간좌외협、간중협공동간체，거간체0．2 cm천간우중협표면고정，결찰간체，우결찰선원측0．2 cm처절제소서간장좌외협、간중협（좌、우）。관찰소서술후성활솔이급술후3d、7d、14d소서간장질량。결과：성공건립소서간장대부분절제모형，해모형절제소서간좌외협、간중협（좌、우），절제간질량점간총질량적66．7％，술후소서존활솔100％。술후소서잉간대상성증생，술후제3천회복지원간총질량적58．2％，제7천회복지원간총질량적78．0％，술후제14천회복지원간총질량적93．2％。결론：본연구건립적개진형소서간장대부분절제후재생모형，구유간편、가조작성강、성공솔고등우점。
Objective:To establish an improved model of mouse partial hepatectomy for study on liver regeneration .Methods :Healthy 8~10 week old male C57BL/6 mice were anesthetized by intraperitoneal injection .Then ,common hepatic pedicle of left lateral lobe of liver and middle lobe of liver was wrapped around with 6-0 proline suture .6-0 proline was fixed on the sur-face of right middle lobe of liver ,which was 0 .2 cm away from the hepatic pedicle .Hepatic pedicle was ligated and left lateral lobe and middle lobe of liver were resected 0 .2 cm away from ligature .The postoperative survival rate and the situation of liver weight vecovery at 3rd day ,7th day ,and 14th day after the operation were observed .Results:An improved mouse model of partial hepatectomy ,of which left lateral lobe of liver and middle lobe of liver were resected ,was established .The weight of removed liver accounted for 66 .7% of total liver and the survival rate of mice was 100% .After liver resection ,the compensa-tory regeneration of remaining liver achieved 58 .2% of the total liver weight at the 3rd day ,78 .0% at the 7th day ,and 93 .2%at the 14th day after the operation .Conclusions :An improved mouse model of partial hepatectomy was successfully established for study on liver regeneration ,which has advantages such as simplicity ,operability and high success rate .
