CAJ | 학술논문

目的：探讨重组人生长激素（rhGH）对肠梗阻大鼠肠黏膜屏障和免疫屏障的保护作用。方法结扎SD大鼠末端回肠，使肠腔狭窄75％，制作成不完全性肠梗阻动物模型；并应用rhGH 6 d （每日1次），观察其回肠黏膜形态学、回肠液sIgA改变及肠道细菌移位情况。结果肠梗阻时，大鼠回肠壁表现为肌层变薄，黏膜厚度减少，肠绒毛变短、变细；回肠液中sIgA浓度减少及细菌移位数量增加；应用rhGH后，回肠黏膜厚度、肠绒毛结构保持相对完整，回肠液中sIgA浓度升高，细菌移位减少。结论肠梗阻时，大鼠肠道黏膜屏障和免疫屏障功能受损，给予外源性rhGH可以较好保护受损肠道的黏膜屏障和免疫屏障功能。
목적：탐토중조인생장격소（rhGH）대장경조대서장점막병장화면역병장적보호작용。방법결찰SD대서말단회장，사장강협착75％，제작성불완전성장경조동물모형；병응용rhGH 6 d （매일1차），관찰기회장점막형태학、회장액sIgA개변급장도세균이위정황。결과장경조시，대서회장벽표현위기층변박，점막후도감소，장융모변단、변세；회장액중sIgA농도감소급세균이위수량증가；응용rhGH후，회장점막후도、장융모결구보지상대완정，회장액중sIgA농도승고，세균이위감소。결론장경조시，대서장도점막병장화면역병장공능수손，급여외원성rhGH가이교호보호수손장도적점막병장화면역병장공능。
Objetive To study of recombinant human growth hormone (rhGH )on intestinal obstruc-tion in the rat intestinal mucosal barrier and immune barrier function. Methods Ligation of the SD rat ileum, the lumen stenosis 75%,made into incomplete intestinal obstruction animal model and the application of rhGH 6 day (once a day),observe the ileal mucosa morphology、ileal fluid sIgA change and bacterial translocation ca-ses. Results Intestinal obstruction,rat ileum wall demonstrated intestinal muscle layer thinning,mucosal thickness reduction of intestinal villi,shorter,thinner;ileal concentration of sIgA in reducing bacterial translo-cation and increased in number;the application of recombinant human growth hormone (rhGH),ileal mucosa thickness,villus structure remained relatively intact,elevated sIgA concentration in ileal fluid and reduced bac-terial translocation. Conclusion Intestinal obstruction,rat intestinal mucosal barrier and immune barrier func-tion is impaired,the exogenous rhGH can better protect the impaired intestinal mucosal barrier and immune bar-rier function.