CAJ | 학술논문

目的：探讨抑癌基因FRK（ Fyn-related kinase ）影响胶质瘤细胞侵袭和迁移的机制。方法将真核表达质粒pcDNA3．0-FRK和对照质粒pcDNA3．0转入人胶质瘤U25l细胞中，western blot技术检测FRK/N-cadherin/E-cadherin蛋白表达，细胞划痕试验检测细胞迁移能力， Transwell 侵袭实验检测细胞侵袭能力。结果与对照组比较，转染pcDNA3．0-FRK质粒24 h后U25l细胞侵袭能力下降47％、迁移能力下降64％，差异均有统计学意义（P ＜0．01）；转染 pcDNA3．0-FRK 可以明显增加 N-cadherin/E-cadherin 的表达。结论FRK可以通过增加N-cadherin/E-cadherin的表达，进而抑制胶质瘤细胞侵袭和迁移能力。
목적：탐토억암기인FRK（ Fyn-related kinase ）영향효질류세포침습화천이적궤제。방법장진핵표체질립pcDNA3．0-FRK화대조질립pcDNA3．0전입인효질류U25l세포중，western blot기술검측FRK/N-cadherin/E-cadherin단백표체，세포화흔시험검측세포천이능력， Transwell 침습실험검측세포침습능력。결과여대조조비교，전염pcDNA3．0-FRK질립24 h후U25l세포침습능력하강47％、천이능력하강64％，차이균유통계학의의（P ＜0．01）；전염 pcDNA3．0-FRK 가이명현증가 N-cadherin/E-cadherin 적표체。결론FRK가이통과증가N-cadherin/E-cadherin적표체，진이억제효질류세포침습화천이능력。
Objective To investigate the effect and mechanism of FRK ( Fyn-related kinase ) on the human glioma cells migration and invasion .Methods Transfection of the pcDNA3.0-FRK plasmid into the U25l glioma cell were carried out using Lipofectamine 2000.Wound healing assay and Transwell invasion assay were used to analyze the influence on the migration and invasion of glioma U251 cells after transfection of the pcDNA 3.0-FRK plasmid.Western blot was used to analyze the protein expressions of E-cadherin/N-cadherin after FRK over-expression .Results Compared to the control plasmids , pcDNA3.0-FRK plasmid could increase the expression of FRK in glioma U 251 cells .In cell invasion assay , FRK inhibited cell invasion ability by 47%.In cell migration assay , FRK inhibited cell migration ability by 64%.Over-expression of FRK led to significant increase of E-cadherin/N-cadherin protein expression levels in glioma U 251 cells.Conclusion FRK may be inhibit glioma cells migration and invasion via increasing the expression of E -cadherin/N-cadherin .