医学研究与教育
醫學研究與教育
의학연구여교육
MEDICAL RESEARCH AND EDUCATION
2014年
3期
1-5
,共5页
董超%马新乔%高军秒%李芸
董超%馬新喬%高軍秒%李蕓
동초%마신교%고군초%리예
血府逐瘀方%动脉粥样硬化%MMP-2%TIMP-2
血府逐瘀方%動脈粥樣硬化%MMP-2%TIMP-2
혈부축어방%동맥죽양경화%MMP-2%TIMP-2
Xuefu Zhuyu Decoction%Atheroselerosis%MMP-2%TIMP-2
目的：通过研究血府逐瘀方对动脉粥样硬化大鼠动脉斑块组织基质金属蛋白酶2（MMP-2）及基质金属蛋白酶抑制因子2（TIMP-2）表达的影响，探讨血府逐瘀方抗动脉粥样硬化作用的可能机制。方法将30只SD大鼠随机分为对照组、模型组、血府逐瘀方组。对照组给予正常饲料，采用腹腔注射维生素D3及饲喂高脂饲料的方法建立大鼠动脉粥样硬化模型。实验结束后，取大鼠主动脉粥样硬化组织检测MMP-2和的TIMP-2的表达水平。结果HE染色光镜下显示，对照组动脉壁形态结构完整，模型组动脉内膜粗糙，中膜平滑肌增生排列紊乱，血府逐瘀方组形态介于二者之间；免疫组化观察，与对照组相比，模型组和血府逐瘀方组MMP-2的表达显著升高，TIMP-2的表达显著下降；血府逐瘀方组与模型组相比，MMP-2表达显著降低，TIMP-2表达显著升高。结论血府逐瘀方能抑制动脉粥样硬化的形成，其机制可能与其能下调动脉粥样硬化大鼠动脉粥样硬化组织内的MMP-2的表达及升高TIMP-2表达有关。
目的：通過研究血府逐瘀方對動脈粥樣硬化大鼠動脈斑塊組織基質金屬蛋白酶2（MMP-2）及基質金屬蛋白酶抑製因子2（TIMP-2）錶達的影響，探討血府逐瘀方抗動脈粥樣硬化作用的可能機製。方法將30隻SD大鼠隨機分為對照組、模型組、血府逐瘀方組。對照組給予正常飼料，採用腹腔註射維生素D3及飼餵高脂飼料的方法建立大鼠動脈粥樣硬化模型。實驗結束後，取大鼠主動脈粥樣硬化組織檢測MMP-2和的TIMP-2的錶達水平。結果HE染色光鏡下顯示，對照組動脈壁形態結構完整，模型組動脈內膜粗糙，中膜平滑肌增生排列紊亂，血府逐瘀方組形態介于二者之間；免疫組化觀察，與對照組相比，模型組和血府逐瘀方組MMP-2的錶達顯著升高，TIMP-2的錶達顯著下降；血府逐瘀方組與模型組相比，MMP-2錶達顯著降低，TIMP-2錶達顯著升高。結論血府逐瘀方能抑製動脈粥樣硬化的形成，其機製可能與其能下調動脈粥樣硬化大鼠動脈粥樣硬化組織內的MMP-2的錶達及升高TIMP-2錶達有關。
목적：통과연구혈부축어방대동맥죽양경화대서동맥반괴조직기질금속단백매2（MMP-2）급기질금속단백매억제인자2（TIMP-2）표체적영향，탐토혈부축어방항동맥죽양경화작용적가능궤제。방법장30지SD대서수궤분위대조조、모형조、혈부축어방조。대조조급여정상사료，채용복강주사유생소D3급사위고지사료적방법건립대서동맥죽양경화모형。실험결속후，취대서주동맥죽양경화조직검측MMP-2화적TIMP-2적표체수평。결과HE염색광경하현시，대조조동맥벽형태결구완정，모형조동맥내막조조，중막평활기증생배렬문란，혈부축어방조형태개우이자지간；면역조화관찰，여대조조상비，모형조화혈부축어방조MMP-2적표체현저승고，TIMP-2적표체현저하강；혈부축어방조여모형조상비，MMP-2표체현저강저，TIMP-2표체현저승고。결론혈부축어방능억제동맥죽양경화적형성，기궤제가능여기능하조동맥죽양경화대서동맥죽양경화조직내적MMP-2적표체급승고TIMP-2표체유관。
Objective To explore mechanism of Xuefu Zhuyu Decoction on the expression of matrix metalloproteianse-2(MMP-2) and tissue metalloproteinase inhibitor -2 (TIMP- 2) in atheroselerotic rats. Methods 30 SD rats were randomly divided into the control group, model group and Xuefu Zhuyu Decoction group. Subcutaneous injection vitD3 with high fat feeding was conducted to reproduce atheroselerotic rats model. After the experiment, immunohistochemistry staining was performed to detect the level of MMP- 2 and TIMP-2 protein expression. Results Under light microscope, arterial structure of the control group was completed, artery intima of the model group was rough, the smooth muscle was disordered arrangement, Xuefu Zhuyu Decoction forms was between the two;Compared with the control group, the expressions of MMP-2 was increased signiifcantly and the expressions of TIMP-2 was decreased signiifcantly in in model group and Xuefu Zhuyu Decoction group. Compared with the model group, the expressions of MMP-2 protein in Xuefu Zhuyu Decoction group were decreased signiifcantly, the expressions of TIMP-2 protein were increased signiifcantly.Conclusion The Xuefu Zhuyu Decoction Could downregulate the expressions of MMP-2 and upregulate he expressions of TIMP-2 and inhibit the formation of atherosclerosis.
