CAJ | 학술논문

仙台病毒（Sendai virus，SeV）是一种常见的可引起啮齿类动物呼吸道疾病的病原，感染迅速，并且隐性感染率高，一旦感染较难从鼠群中清除，从而影响动物健康。为满足对入境噬齿类实验动物和野生动物仙台病毒快速检测的需要，本研究针对SeV编码基质蛋白和融合糖蛋白基因的保守序列设计引物和荧光探针，建立了仙台病毒RT-PCR和Real-time RT-PCR检测方法。将建立的方法应用于仙台病毒感染鼠不同临床样品和组织培养物的检测，证实两种核酸检测方法具有良好的特异性、灵敏性和稳定性，适合应用于出入境口岸实验和野生噬齿类动物仙台病毒疫情的快速检测。
선태병독（Sendai virus，SeV）시일충상견적가인기교치류동물호흡도질병적병원，감염신속，병차은성감염솔고，일단감염교난종서군중청제，종이영향동물건강。위만족대입경서치류실험동물화야생동물선태병독쾌속검측적수요，본연구침대SeV편마기질단백화융합당단백기인적보수서렬설계인물화형광탐침，건립료선태병독RT-PCR화Real-time RT-PCR검측방법。장건립적방법응용우선태병독감염서불동림상양품화조직배양물적검측，증실량충핵산검측방법구유량호적특이성、령민성화은정성，괄합응용우출입경구안실험화야생서치류동물선태병독역정적쾌속검측。
Sendai virus (SeV) is one of common pathogens causing respiratory disease among rodent animals, which is characterized by rapid infection and high recessive infection rates. Therefore, SeV infection is difficult to eliminate from affected animals and might interfere with animal experiments. In order to meet requirements for rapid quarantine for rodent animals, RT-PCR and Real-time RT-PCR methods were developed using TaqMan probe to detect SeV. Both methods were applied to test clinical samples collected from SeV infected mice and cell cultures. The results showed that RT-PCR and Real-time RT-PCR methods were specific, sensitive and reproducible thus both methods were suitable for diagnosis of SeV infection in rodent animals.