中医药信息
中醫藥信息
중의약신식
INFORMATION ON TRADITIONAL CHINESE MEDICINE
2014年
4期
107-111
,共5页
莫雨平%邓晓丰%纪倩%滕金艳%姚海江%宋洪涛%唐银杉%许安萍%张淑静%李志刚
莫雨平%鄧曉豐%紀倩%滕金豔%姚海江%宋洪濤%唐銀杉%許安萍%張淑靜%李誌剛
막우평%산효봉%기천%등금염%요해강%송홍도%당은삼%허안평%장숙정%리지강
电针%慢性应激%抑郁症%大鼠%海马%血清%细胞因子
電針%慢性應激%抑鬱癥%大鼠%海馬%血清%細胞因子
전침%만성응격%억욱증%대서%해마%혈청%세포인자
Electro-acupuncture%Chronic unpredictable mild stress%Depression%Rats%Hippocampus%Serum%Cytokine
目的：观察不同电针对慢性应激抑郁模型（ chronic unpredictable mild stress ，CUMS）大鼠海马及血清中细胞因子IL-1β、IL-6表达的影响，探讨IL-1β、IL-6在大鼠实验性抑郁症发病过程中的作用机制及不同电针干预对其影响。方法：将雄性清洁健康级SD大鼠60只，随机分为正常组、模型组、电针组、音乐电针组及百忧解组，每组12只。孤养结合CUMS 21天复制抑郁症模型，通过开野试验观察大鼠行为学的变化，采用免疫组化染色、酶联免疫吸附法分别检测IL-1β、IL-6在大鼠海马及血清中的表达情况。结果：模型组大鼠开野实验水平运动及垂直运动明显减少，电针组、音乐电针组及百忧解组均可改善此情况；模型组大鼠血清及海马内IL-1β、IL-6表达均明显升高，电针组、音乐电针组及百忧解组可逆转此病理变化，但三者之间无显著性差异。结论：抑郁大鼠的IL-1β、IL-6含量高于正常组；电针治疗抑郁症可能是通过降低IL-1β、IL-6的含量发挥抗抑郁作用。音乐电针与普通电针治疗抑郁症效果差异不明显。
目的：觀察不同電針對慢性應激抑鬱模型（ chronic unpredictable mild stress ，CUMS）大鼠海馬及血清中細胞因子IL-1β、IL-6錶達的影響，探討IL-1β、IL-6在大鼠實驗性抑鬱癥髮病過程中的作用機製及不同電針榦預對其影響。方法：將雄性清潔健康級SD大鼠60隻，隨機分為正常組、模型組、電針組、音樂電針組及百憂解組，每組12隻。孤養結閤CUMS 21天複製抑鬱癥模型，通過開野試驗觀察大鼠行為學的變化，採用免疫組化染色、酶聯免疫吸附法分彆檢測IL-1β、IL-6在大鼠海馬及血清中的錶達情況。結果：模型組大鼠開野實驗水平運動及垂直運動明顯減少，電針組、音樂電針組及百憂解組均可改善此情況；模型組大鼠血清及海馬內IL-1β、IL-6錶達均明顯升高，電針組、音樂電針組及百憂解組可逆轉此病理變化，但三者之間無顯著性差異。結論：抑鬱大鼠的IL-1β、IL-6含量高于正常組；電針治療抑鬱癥可能是通過降低IL-1β、IL-6的含量髮揮抗抑鬱作用。音樂電針與普通電針治療抑鬱癥效果差異不明顯。
목적：관찰불동전침대만성응격억욱모형（ chronic unpredictable mild stress ，CUMS）대서해마급혈청중세포인자IL-1β、IL-6표체적영향，탐토IL-1β、IL-6재대서실험성억욱증발병과정중적작용궤제급불동전침간예대기영향。방법：장웅성청길건강급SD대서60지，수궤분위정상조、모형조、전침조、음악전침조급백우해조，매조12지。고양결합CUMS 21천복제억욱증모형，통과개야시험관찰대서행위학적변화，채용면역조화염색、매련면역흡부법분별검측IL-1β、IL-6재대서해마급혈청중적표체정황。결과：모형조대서개야실험수평운동급수직운동명현감소，전침조、음악전침조급백우해조균가개선차정황；모형조대서혈청급해마내IL-1β、IL-6표체균명현승고，전침조、음악전침조급백우해조가역전차병리변화，단삼자지간무현저성차이。결론：억욱대서적IL-1β、IL-6함량고우정상조；전침치료억욱증가능시통과강저IL-1β、IL-6적함량발휘항억욱작용。음악전침여보통전침치료억욱증효과차이불명현。
Objective:To investigate the effect on the expression of IL -1β, IL-6 in the hippocampus and ser-um of rats with chronic unpredictable mild stress treated by different electro -acupuncture(EA) treatment, to elucidate the possible role of IL -1β, IL-6 in the development of experimental depression and the effect of different EA for them .Methods:Adult male SD rats were randomly divided into normal control group ( n=12 ) , model group ( n=12 ) , pulsed EA group ( n=12 ) , music EA group ( n=12 ) and fluoxetine group ( n=12 ) . Depressive models were established by lonely raising and chronic unpredictable mild stress for 21 days. Open-field test was performed to test the behavior of rats .The expression of hippocampus and serum IL -1β, IL-6 was detected by using immunohistochemistry and enzyme linked immunosorbent assay ( ELISA ) techniques respectively .Results:The cross and rear score of model group was significantly lower than that in normal control group , while pulsed EA group , music EA group and fluoxetine group could reverse it .The ex-pression of hippocampus and serum IL -1β, IL-6 in model group increased significantly .The three kinds of treatment could improve these pathological change , but there was no difference among these effects of treat-ment .Conclusion:The levels of IL-1β, IL-6 in hippocampus and serum of depressive rats are higher than those in the normal group .EA treatment may have antagonism against depression by lowering the levels of IL-1β, IL-6 in hippocampus and serum ,and there is no difference between the effects of pulsed EA treat-ment and music EA treatment for depression .
