中医药信息
中醫藥信息
중의약신식
INFORMATION ON TRADITIONAL CHINESE MEDICINE
2014年
4期
98-101
,共4页
郭乐%周赛男%蔺晓源%易健%郭纯%刘柏炎%蔡光先
郭樂%週賽男%藺曉源%易健%郭純%劉柏炎%蔡光先
곽악%주새남%린효원%역건%곽순%류백염%채광선
脑缺血损伤%补阳还五汤%神经功能%核转录因子%大鼠
腦缺血損傷%補暘還五湯%神經功能%覈轉錄因子%大鼠
뇌결혈손상%보양환오탕%신경공능%핵전록인자%대서
Cerebral ischemia%Buyang Huanwu decoction%Neurological function%NF-κB%Rats
目的：探讨补阳还五汤对脑缺血后大鼠脑组织NF-κB/p50表达的影响。方法：将180只SD大鼠随机分为空白组、假手术组、模型组、补阳还五汤组、PDTC（呲咯烷二硫代氨基甲酸盐）组和米诺环素组，各30只，每组按给药后7天、14天、21天3个时间点再各分为3个亚组，观察各组不同时间点的神经功能评分，免疫组化检测缺血脑组织NF-κB/p50蛋白表达情况。结果：补阳还五汤组、米诺环素组、PDTC组各时间点神经功能缺失症状评分和脑组织NF-κB/p50阳性细胞数与模型组比较，均明显减少（P＜0．05或P＜0．01）；用药7天、14天后，补阳还五汤组NF-κB/p50阳性细胞数与米诺环素组，PDTC组组间差异无统计学意义（P＞0.05），用药21天后，补阳还五汤组与PDTC组比较，具有显著性差异（P＜0．05）。结论：补阳还五汤可促进脑缺血损伤后神经功能恢复，调控缺血脑组织炎症因子NF-κB/p50的表达可能是其治疗脑缺血的作用机制之一。
目的：探討補暘還五湯對腦缺血後大鼠腦組織NF-κB/p50錶達的影響。方法：將180隻SD大鼠隨機分為空白組、假手術組、模型組、補暘還五湯組、PDTC（呲咯烷二硫代氨基甲痠鹽）組和米諾環素組，各30隻，每組按給藥後7天、14天、21天3箇時間點再各分為3箇亞組，觀察各組不同時間點的神經功能評分，免疫組化檢測缺血腦組織NF-κB/p50蛋白錶達情況。結果：補暘還五湯組、米諾環素組、PDTC組各時間點神經功能缺失癥狀評分和腦組織NF-κB/p50暘性細胞數與模型組比較，均明顯減少（P＜0．05或P＜0．01）；用藥7天、14天後，補暘還五湯組NF-κB/p50暘性細胞數與米諾環素組，PDTC組組間差異無統計學意義（P＞0.05），用藥21天後，補暘還五湯組與PDTC組比較，具有顯著性差異（P＜0．05）。結論：補暘還五湯可促進腦缺血損傷後神經功能恢複，調控缺血腦組織炎癥因子NF-κB/p50的錶達可能是其治療腦缺血的作用機製之一。
목적：탐토보양환오탕대뇌결혈후대서뇌조직NF-κB/p50표체적영향。방법：장180지SD대서수궤분위공백조、가수술조、모형조、보양환오탕조、PDTC（자각완이류대안기갑산염）조화미낙배소조，각30지，매조안급약후7천、14천、21천3개시간점재각분위3개아조，관찰각조불동시간점적신경공능평분，면역조화검측결혈뇌조직NF-κB/p50단백표체정황。결과：보양환오탕조、미낙배소조、PDTC조각시간점신경공능결실증상평분화뇌조직NF-κB/p50양성세포수여모형조비교，균명현감소（P＜0．05혹P＜0．01）；용약7천、14천후，보양환오탕조NF-κB/p50양성세포수여미낙배소조，PDTC조조간차이무통계학의의（P＞0.05），용약21천후，보양환오탕조여PDTC조비교，구유현저성차이（P＜0．05）。결론：보양환오탕가촉진뇌결혈손상후신경공능회복，조공결혈뇌조직염증인자NF-κB/p50적표체가능시기치료뇌결혈적작용궤제지일。
Objective:To observe the effect of Buyang Huanwu decoction on NF -κB/p50 expression in brain tissue of rats with focal cerebral ischemia injury .Methods:One hundred and eighty SD rats were randomly di-vided into normal group , Sham -operated group , ischemia group , BYHWD treatment group , PDTC treatment group and MC treatment group .Each group was divided into three subgroups according to the time after admin-istration for 7d,14d,21d respectively.Neurological function score and NF -κB/p50 protein levels were exam-ined by using immunohistochemistry in each group at different time points .Results:Neurological function score and expression of NF-κB/p50 in BYHWD,MC,PDTC treatment group were significantly decreased compared with those in model group at the same time (P<0.05 or P<0.01);after 7,14 days’treatment, the difference of the expression of NF -κB/p50 was not significant ( P>0.05) .Between the treatment groups ,after 21 days’ treatment BYHWD displayed the trifle superiority in comparison with PDTC ( P<0 .05 ) .Conclusion:Buyang Huanwu decoction can improve the recovery of cerebral ischemic neurological injury and regulating cerebral in -flammatory cytokine NF-κB/p50 expression of ischemia rats may be one of its therapeutic mechanisms for cerebral ischemia .
