CAJ | 학술논문

目的：通过对桦褐孔菌与玉米须双向固体发酵工艺的研究，优化其培养条件。方法：采用固体发酵方法得到发酵产物，通过监测菌丝体生长速度获得平均生长速率、通过紫外分光光度法进行总多糖及总三萜含量测定，最后运用加权平均法确定最佳培养条件。结果：最佳培养条件为培养温度（28±2）℃，固体物料玉米须与玉米芯的最佳配比为1∶3，培养料干重90g/袋，液体菌种接种量为2ml/袋，静置培养周期为50天。结论：通过双向固体发酵技术，采用不同配比固体培养基得到的桦须菌质（ HXJZ），菌丝平均生长速率趋势平稳，总多糖、总三萜生物转化率稳定，能够从一定程度上改善桦褐孔菌资源贫瘠的现状，为新药源的开发奠定基础。
목적：통과대화갈공균여옥미수쌍향고체발효공예적연구，우화기배양조건。방법：채용고체발효방법득도발효산물，통과감측균사체생장속도획득평균생장속솔、통과자외분광광도법진행총다당급총삼첩함량측정，최후운용가권평균법학정최가배양조건。결과：최가배양조건위배양온도（28±2）℃，고체물료옥미수여옥미심적최가배비위1∶3，배양료간중90g/대，액체균충접충량위2ml/대，정치배양주기위50천。결론：통과쌍향고체발효기술，채용불동배비고체배양기득도적화수균질（ HXJZ），균사평균생장속솔추세평은，총다당、총삼첩생물전화솔은정，능구종일정정도상개선화갈공균자원빈척적현상，위신약원적개발전정기출。
Objective:To research the cultivating condition on bothway solid fermentation conditions of Inonotus obliquus for transforming Chinese medicine Stigma maydis in order to increase the conversion rate .Method:The fermentation products were achieved by solid fermentation method .The average growth rate ,the content of total polysaccharides and total triterpenoid were observed respectively by mycelium growth rate and by ultravio -let spectroscopy , at last use the weighted average method to determine the best cultivating condition .Results:The best cultivating condition , the cultivating temperature ( 28 ±2 )℃, the best ratio of solid material of Stig-ma and Corn cob was 1∶3, substrates of dry weight and wet weight were 90g/bag.The liquid inoculation a-mount was 2ml/bag and culturing circle was 50d.Conclusion:Using bothway solid fermentation condition tech-nology ,through different proportion solid medium to obtain HXJZ , the trend of average growth rate of mycelium was stationary , the rate of transforming total polysaccharides and total triterpenoid were stable ,to a certain ex-tent,it can improve the state of the resource -poor of Inonotus obliquus and lays a foundation for the develop-ment of new pharmacy .