CAJ | 학술논문

目的：建立贝母花的定性鉴别和含量测定方法。方法：采用薄层色谱法进行定性鉴别；ELSD-HPLC法测定贝母素甲和贝母素乙的含量，采用Agilent 1260高效液相色谱仪， Agilent 380蒸发光散射检测器检测，C18色谱柱（5μm，4．6mm ×150mm，）；流动相：乙腈-水-三乙胺（68∶32∶0．03），流速：1.0 mL/min；柱温：30℃；漂移管温度：90℃；进样量：20μL。结果：薄层色谱专属性强；贝母素甲、贝母素乙的线性范围分别是0．0185～0．185mg/mL（r＝0．9998），0．0188～0．188mg/mL（r＝0.9997）；平均加样回收率为100．40％，RSD为1．50％（n＝6）。结论：该方法简便、准确，重现性好，可作为控制贝母花质量的方法。
목적：건립패모화적정성감별화함량측정방법。방법：채용박층색보법진행정성감별；ELSD-HPLC법측정패모소갑화패모소을적함량，채용Agilent 1260고효액상색보의， Agilent 380증발광산사검측기검측，C18색보주（5μm，4．6mm ×150mm，）；류동상：을정-수-삼을알（68∶32∶0．03），류속：1.0 mL/min；주온：30℃；표이관온도：90℃；진양량：20μL。결과：박층색보전속성강；패모소갑、패모소을적선성범위분별시0．0185～0．185mg/mL（r＝0．9998），0．0188～0．188mg/mL（r＝0.9997）；평균가양회수솔위100．40％，RSD위1．50％（n＝6）。결론：해방법간편、준학，중현성호，가작위공제패모화질량적방법。
To establish the quality standard for flowers of Fritillaria thunbergii Miq.Methods:Flowers of Fritillaria thunbergii Miq.were identified by TLC .The contents of peimine and peiminninne were determined by ELSD-HPLC:The optimal conditions of separation and detection were achieved on a C18 ana-lytical column with an mobile phase consisting of acetonitrile -water -triethylamine ( 68∶32∶0 .03 ) at the flow-rate of 1.0 mL/min;the temperature for the detector drift tube was set at 90℃and the nitrogen flow-rate was at 2.2L/min, 20μL as the injection volume .Results:The identification by TLC was highly specific . The calibration curves for peimine and peiminine were linear over the range of 0 .0185~0 .185 mg/mL (r=0.9998 ) and 0.0188~0.188 mg/mL(r=0.9997) , respectively .The average recovery rate was 100.40%with RSD of 1.50%(n=6).Conclusion:The method is simple and reproducible . It can serve as a quantita-tive determination and quality control of flowers of Fritillaria thunbergii Miq.