中医药信息
中醫藥信息
중의약신식
INFORMATION ON TRADITIONAL CHINESE MEDICINE
2014年
4期
14-16
,共3页
吴勃岩%王雪%董静%刘文波%孙阳%郭艳莹%王晓军
吳勃巖%王雪%董靜%劉文波%孫暘%郭豔瑩%王曉軍
오발암%왕설%동정%류문파%손양%곽염형%왕효군
齐墩果酸%细胞凋亡%细胞色素C%Caspase-3%抗肿瘤
齊墩果痠%細胞凋亡%細胞色素C%Caspase-3%抗腫瘤
제돈과산%세포조망%세포색소C%Caspase-3%항종류
Oleanolic acid%Cell apoptosis%Cytochrome C%Caspase-3%Antitumor
目的：探讨齐墩果酸诱导荷瘤小鼠肿瘤细胞凋亡作用机制。方法：将接种S180腹水瘤的小鼠随机分为模型组，齐墩果酸高、中、低剂量组，环磷酰胺组。采用免疫组化法检测肿瘤组织中细胞色素C和Caspase-3蛋白表达水平。结果：齐墩果酸对S180荷瘤小鼠细胞色素C和Caspase -3蛋白表达水平明显提高，与模型组比较差异显著（P＜0．01），具有诱导肿瘤细胞凋亡作用。结论：齐墩果酸能上调S180荷瘤小鼠肿瘤细胞内细胞色素C和Caspase-3蛋白的表达，通过线粒体凋亡通路诱导肿瘤细胞凋亡而发挥抗肿瘤作用。
目的：探討齊墩果痠誘導荷瘤小鼠腫瘤細胞凋亡作用機製。方法：將接種S180腹水瘤的小鼠隨機分為模型組，齊墩果痠高、中、低劑量組，環燐酰胺組。採用免疫組化法檢測腫瘤組織中細胞色素C和Caspase-3蛋白錶達水平。結果：齊墩果痠對S180荷瘤小鼠細胞色素C和Caspase -3蛋白錶達水平明顯提高，與模型組比較差異顯著（P＜0．01），具有誘導腫瘤細胞凋亡作用。結論：齊墩果痠能上調S180荷瘤小鼠腫瘤細胞內細胞色素C和Caspase-3蛋白的錶達，通過線粒體凋亡通路誘導腫瘤細胞凋亡而髮揮抗腫瘤作用。
목적：탐토제돈과산유도하류소서종류세포조망작용궤제。방법：장접충S180복수류적소서수궤분위모형조，제돈과산고、중、저제량조，배린선알조。채용면역조화법검측종류조직중세포색소C화Caspase-3단백표체수평。결과：제돈과산대S180하류소서세포색소C화Caspase -3단백표체수평명현제고，여모형조비교차이현저（P＜0．01），구유유도종류세포조망작용。결론：제돈과산능상조S180하류소서종류세포내세포색소C화Caspase-3단백적표체，통과선립체조망통로유도종류세포조망이발휘항종류작용。
Objective:The mechanism of cell apoptosis had been explored after tumor -bearing mice were trea-ted by oleanolic acid.Methods: There were five groups of S180 tumor -bearing mice, which were model group, oleanolic acid groups with different doses ( high, middle and low ) and cyclophosphamide group .The expressions of cytochrome C and Caspase -3 were measured by immunohistochemistry .Results: Oleanolic acid induced the protein level of cytochrome C and Caspase -3 increased compared with the model group ( P<0.01 ) .Oleanolic acid may induce the tumor cell apoptosis .Conclusion:Oleanolic acid can up-regulate the protein expressions of cytochrome C and Caspase -3 of S180 tumor-bearing mice .It is through mitochondrial apoptotic pathway for exerting antitumor effect .
