CAJ | 학술논문

石斑鱼类（Groupers）是世界性海洋经济鱼类，也是中国南方沿海省份重要的海水增养殖对象，其种类繁多，由于缺乏明显的种间外部形态区别特征，石斑鱼的分类一直是鱼类系统分类学的一个难题，存在着较多的争议和混淆。为了从分子水平揭示石斑鱼类的系统进化关系，应用线粒体细胞色素b基因（ Cyt b ）及两个核糖体RNA基因（16S和12S）序列联合构建系统进化树对中国近海石斑鱼亚科3属13种进行种类鉴定及亲缘关系分析，探讨了石斑鱼类的分子系统进化。结果表明：①线粒体基因序列在石斑鱼类种间存在较大的变异，种内变异极小，是进行石斑鱼类种类鉴定较为适宜的分子标记；②所研究的3属13种石斑鱼类中，石斑鱼属与九棘鲈属的亲缘关系较近，与鳃棘鲈属的较远；③赤点石斑鱼和青石斑鱼、蜂巢石斑鱼与鮭点石斑鱼、斜带石斑鱼与棕斑石斑鱼两两间具有较近的亲缘关系。
석반어류（Groupers）시세계성해양경제어류，야시중국남방연해성빈중요적해수증양식대상，기충류번다，유우결핍명현적충간외부형태구별특정，석반어적분류일직시어류계통분류학적일개난제，존재착교다적쟁의화혼효。위료종분자수평게시석반어류적계통진화관계，응용선립체세포색소b기인（ Cyt b ）급량개핵당체RNA기인（16S화12S）서렬연합구건계통진화수대중국근해석반어아과3속13충진행충류감정급친연관계분석，탐토료석반어류적분자계통진화。결과표명：①선립체기인서렬재석반어류충간존재교대적변이，충내변이겁소，시진행석반어류충류감정교위괄의적분자표기；②소연구적3속13충석반어류중，석반어속여구극로속적친연관계교근，여새극로속적교원；③적점석반어화청석반어、봉소석반어여해점석반어、사대석반어여종반석반어량량간구유교근적친연관계。
The groupers are commercially important marine species over the world , and they are also the important mariculture species of the coastal provinces in south China .The great variety of the species and the lack of morphological characteristics led to much confusion and dispution in their phylogenetic rela-tionships and species identification .In the present study , we try to reveal the phylogenetic relationships of groupers on molecular level .The partial mitochondrial cytochrome b ( Cyt b ) gene and two ribosomal genes (16S and 12S) were combined and applied to species identification and relationship analysis of 13 Epinepheline species from 3 genera distributed in coastal waters of south China .The results showed that:①The mitochondrial genes are appropriate as molecular markers for Epinepheline species identification because considerably high sequence difference among species and very low within species were found in these 3 genera;② Epinephelus was more closely related to Cephalopholis than to Plectropomus; ③ The genetic relationship was very close between E.akaara and E.awoara, E.merra and E.longispinis, E.coioides and E.corallicola.