CAJ | 학술논문

为有效地研究烟草遗传多样性和筛选潜在的繁殖材料,以贵州省烟草科学研究院805份烟草种质为实验材料,分别在贵州省金沙和福泉2个试验点进行多点田间试验,并采用混合线性模型预测12个农艺性状的基因型效应值,以构建烟草的核心种质库并研究其遗传多样性。结果表明,待预测的12个农艺性状中,11个性状有显著的基因型效应,10个性状有显著的基因型与环境互作效应。利用预测的基因型值,比较不同抽样方法、抽样比率和系统聚类方法下核心子集多样性的结果表明,在10％抽样比率下,重心法聚类结合优先取样获得的S1C3_10子集成为烟草种质库的核心子集。通过主成分分析法比较核心种质与原始群体样本分布的空间结构和特征,运用相关系数检验原始群体性状间的遗传关系是否被核心种质很好地保留。结果表明,核心种质库能很好地代表烟草农艺性状的遗传多样性。一些潜在的高产育种材料如Y177和Y178也被包含在内。综上表明,作为种质资源的一个核心子集,烟草核心种质库的构建将有利于烟草育种者对整个资源的了解,促进遗传多样性在今后烟草育种计划中的利用。
위유효지연구연초유전다양성화사선잠재적번식재료,이귀주성연초과학연구원805빈연초충질위실험재료,분별재귀주성금사화복천2개시험점진행다점전간시험,병채용혼합선성모형예측12개농예성상적기인형효응치,이구건연초적핵심충질고병연구기유전다양성。결과표명,대예측적12개농예성상중,11개성상유현저적기인형효응,10개성상유현저적기인형여배경호작효응。이용예측적기인형치,비교불동추양방법、추양비솔화계통취류방법하핵심자집다양성적결과표명,재10％추양비솔하,중심법취류결합우선취양획득적S1C3_10자집성위연초충질고적핵심자집。통과주성분분석법비교핵심충질여원시군체양본분포적공간결구화특정,운용상관계수검험원시군체성상간적유전관계시부피핵심충질흔호지보류。결과표명,핵심충질고능흔호지대표연초농예성상적유전다양성。일사잠재적고산육충재료여Y177화Y178야피포함재내。종상표명,작위충질자원적일개핵심자집,연초핵심충질고적구건장유리우연초육충자대정개자원적료해,촉진유전다양성재금후연초육충계화중적이용。
Plant germplasm is an important gene resource for plant breeding and genetic improvement of traits . Core collection , as a representative subset of germplasm , provides an effective entry to investigate genetic diversity and screen potential breeding material . Most agronomic traits of tobacco often exhibit strong genetic variation which can be decomposed into genotypic and genotype × environment interaction variations . A tobacco collection of 805 accessions were evaluated in two locations , Fuquan and Jinsha City of Guizhou Province , China . The genetic variations of 12 agronomic traits were analyzed and the genotypic values of each accession were predicted by a mixed linear model approach . Significant genotypic variations were detected for 11 traits , as well as genotype × environment interaction for 10 traits . Based on the predicted genotypic values , the combination strategies of sampling and clustering methods in the procedure of stepwise clustering for constructing core collection were screened in terms of magnitude of genetic variation captured by sampled subsets . Finally , the subset ( S1C3_10) , sampled by a centroid method combined with preferred sampling at 10% proportion , was determined to be a core collection of the tobacco germplasm . The representative and validation of the core collection was examined visually by the accession distribution pattern in the plot established by first two principal components , as well as by the correlation coefficients in terms of magnitude and significance between the core and the initial collections . The results showed that the initial collection was well represented by the core collection , and some potential breeding materials for high yield breeding program of tobacco , such as Y 177 and Y178 , were included in the core collection . In sum , the constructed core collection will facilitate tobacco breeder to access to whole resources and promote the utilization of genetic diversity in future breeding program of tobacco in China .