CAJ | 학술논문

目前，遗传连锁图谱构建主要基于两纯系亲本交配的衍生群体，存在遗传多样性不足等局限，不适合于烟草等基因组多样性较低的物种，因为往往没有足够的多态性标记可用于图谱的构图，稀疏的标记图谱很难有效用于标记辅助选择及基因定位。本研究提出了基于四交群体的遗传图谱构建方法，通过计算机模拟验证了该方法的可行性和有效性，研制了配套的计算机分析程序。该方法的主要步骤及特点如下：1)基于最大似然法估计成对标记间的遗传距离；2)基于标记距离矩阵进行聚类分析，将标记分成不同的类群(连锁群)；3)对每一连锁群，先确定连锁群两端的标记，再通过3标记间遗传距离的比较，将其他标记逐步插入到合适的排列位置，最终确定整个连锁群的标记顺序。蒙特卡洛模拟证实了该方法可行、有效，且适用于其他2个自交系亲本交配的衍生群体。
목전，유전련쇄도보구건주요기우량순계친본교배적연생군체，존재유전다양성불족등국한，불괄합우연초등기인조다양성교저적물충，인위왕왕몰유족구적다태성표기가용우도보적구도，희소적표기도보흔난유효용우표기보조선택급기인정위。본연구제출료기우사교군체적유전도보구건방법，통과계산궤모의험증료해방법적가행성화유효성，연제료배투적계산궤분석정서。해방법적주요보취급특점여하：1)기우최대사연법고계성대표기간적유전거리；2)기우표기거리구진진행취류분석，장표기분성불동적류군(련쇄군)；3)대매일련쇄군，선학정련쇄군량단적표기，재통과3표기간유전거리적비교，장기타표기축보삽입도합괄적배렬위치，최종학정정개련쇄군적표기순서。몽특잡락모의증실료해방법가행、유효，차괄용우기타2개자교계친본교배적연생군체。
Currently , developing genetic linkage map mostly use the derived-populations from crossing of two homogenous parents , which only covers limited genetic diversity and is inappropriate for some species , such as tobacco with lower diversity in genome . It is very general that there are no sufficient polymorphic markers to construct linkage map and ineffective to conduct marker-assisted selection ( M AS ) and quantitative trait locus ( QTL) mapping based on lower density linkage map . This study proposed a method for developing genetic linkage map based on a four-way cross population . Computer simulation was conducted to investigate the feasibility and effectiveness of the method and a supporting program was designed . The main procedures and features of the proposed method were summarized as follows: 1) estimating genetic distance of any paired markers based on maximum likelihood method;2) splitting all markers into different groups ( linkage group) by cluster analysis based on genetic distance of markers; 3) for each linkage group , two end markers were first determined , then the marker order could be determined by inserting other markers in appropriate position by distance analysis of any three neighboring markers . Monte Carlo simulation showed that the proposed method is feasible , effective , and applicable in other derived populations from crossing of two homogenous parents .