浙江大学学报（农业与生命科学版）
浙江大學學報（農業與生命科學版）
절강대학학보（농업여생명과학판）
JOURNAL OF ZHEJIANG UNIVERSITY(AGRICULTURE & LIFE SCIENCES)
2014年
4期
370-378
,共9页
沈恩惠%刘扬%叶楚玉%樊龙江
瀋恩惠%劉颺%葉楚玉%樊龍江
침은혜%류양%협초옥%번룡강
微RNA%小干扰RNA%相位排列的小干扰RNA%内源诱捕靶标%生物信息学
微RNA%小榦擾RNA%相位排列的小榦擾RNA%內源誘捕靶標%生物信息學
미RNA%소간우RNA%상위배렬적소간우RNA%내원유포파표%생물신식학
microRNA%small interfering RNA%phased small interfering RNA%endogenous target mimics%bioinformatics
在植物和动物中存在着大量非编码小RNA ,它们通过对靶标mRNA直接切割或在转录后抑制其翻译对基因表达起调控作用.本文主要综述了该领域在以下2方面的研究进展.1)微RNA(miRNA)介导的具有相位排列的小干扰RNA( phasiRNA)：由miRNA介导的phasiRNA可由编码和非编码位点产生；介绍了phasiRNA产生的模式特征,以及 phasiRNA和miRNA的进化机制.2)介绍了最近出现的内源miRNA诱捕靶标( endogenous target mimics ,eTMs)研究进展,包括利用人工诱捕靶标检验miRNA的功能,通过生物信息学方法在全基因组水平计算识别eT M s等.
在植物和動物中存在著大量非編碼小RNA ,它們通過對靶標mRNA直接切割或在轉錄後抑製其翻譯對基因錶達起調控作用.本文主要綜述瞭該領域在以下2方麵的研究進展.1)微RNA(miRNA)介導的具有相位排列的小榦擾RNA( phasiRNA)：由miRNA介導的phasiRNA可由編碼和非編碼位點產生；介紹瞭phasiRNA產生的模式特徵,以及 phasiRNA和miRNA的進化機製.2)介紹瞭最近齣現的內源miRNA誘捕靶標( endogenous target mimics ,eTMs)研究進展,包括利用人工誘捕靶標檢驗miRNA的功能,通過生物信息學方法在全基因組水平計算識彆eT M s等.
재식물화동물중존재착대량비편마소RNA ,타문통과대파표mRNA직접절할혹재전록후억제기번역대기인표체기조공작용.본문주요종술료해영역재이하2방면적연구진전.1)미RNA(miRNA)개도적구유상위배렬적소간우RNA( phasiRNA)：유miRNA개도적phasiRNA가유편마화비편마위점산생；개소료phasiRNA산생적모식특정,이급 phasiRNA화miRNA적진화궤제.2)개소료최근출현적내원miRNA유포파표( endogenous target mimics ,eTMs)연구진전,포괄이용인공유포파표검험miRNA적공능,통과생물신식학방법재전기인조수평계산식별eT M s등.
There are many non-coding small RNAs in plants and animals , which regulate gene expression via direct cleavage of target mRNAs or via inhibition of translation at the post-transcriptional level . In this paper , recent studies on microRNA ( miRNA )-mediated phased siRNAs ( phasiRNA ) and endogenous target mimics (eTMs) were reviewed:1) phasiRNAs can be generated both in coding and non-coding loci , and some require miRNA-mediated cleavage for their biogenesis . Pattern and evolutionary mechanisms of miRNA-mediated phasiRNAs were discussed . 2) Genome-wide identification and application of eTMs as miRNA decoy targets were recommended , including the use of artificial target mimics to validate the functions of miRNAs and employing bioinformatics methods to identify eTMs in the whole-genome level .
