中华消化病与影像杂志（电子版）
中華消化病與影像雜誌（電子版）
중화소화병여영상잡지（전자판）
2014年
4期
183-188
,共6页
孙小雅%张海燕%刘立新%郭晓红
孫小雅%張海燕%劉立新%郭曉紅
손소아%장해연%류립신%곽효홍
肝硬化%胰岛素样生长因子结合蛋白质 1%NF-κB%腺病毒科%大鼠, Sprague-Dawley
肝硬化%胰島素樣生長因子結閤蛋白質 1%NF-κB%腺病毒科%大鼠, Sprague-Dawley
간경화%이도소양생장인자결합단백질 1%NF-κB%선병독과%대서, Sprague-Dawley
Liver cirrhosis%Insulin -like growth factor binding protein 1%NF-kappa B%Adenoviridae%Rats,Sprague-Dawley
目的：探讨腺病毒包装的胰岛素样生长因子结合蛋白相关蛋白1（ Ad-IGFBPrP1）对大鼠肝组织中NF-κB p65的表达影响及意义。方法32只健康雄性SD大鼠随机分为4组，每组8只。正常对照组：尾静脉注射生理盐水；阴性对照Ad-EGFP组：尾静脉注射腺病毒包装的增强型绿色荧光蛋白（ Ad-EGFP）；Ad-IGFBPrP12周组：尾静脉注射 Ad-IGFBPrP1；Ad-IGFBPrP14周组：尾静脉注射Ad-IGFBPrP1。分别于注射2周、4周末乙醚麻醉大鼠，取肝左叶固定、包埋、切片。行HE染色，观察肝组织病理改变；苦味酸-天狼星红染色观察肝组织中胶原纤维含量的变化；免疫组织化学染色观察IGFBPrP1、核因子κBp65（NF-κBp65）、α-平滑肌肌动蛋白（α-SMA）、纤维连接蛋白（Fn）的表达及分布。结果 HE和苦味酸-天狼星红染色显示：与正常对照组、阴性对照Ad-EGFP组的正常肝组织相比，Ad-IGFBPrP1组肝组织出现病理改变且发生纤维化（ P ＜0.01）。免疫组织化学染色显示：Ad-IGFBPrP1组肝组织中IGFBPrP1、NF-κB p65、α-SMA、Fn的表达较正常对照组、阴性对照Ad-EGFP组明显增强（ P＜0.01）；Ad-IGFBPrP14周组的表达高于2周组（ P＜0.01）。结论 Ad-IGFBPrP1经尾静脉注射成功转染进入大鼠，引起肝组织中NF-κB p65表达增高，最终导致肝组织纤维化，提示IGFBPrP1致肝纤维化的机制之一可能通过NF-κB信号转导通路实现。
目的：探討腺病毒包裝的胰島素樣生長因子結閤蛋白相關蛋白1（ Ad-IGFBPrP1）對大鼠肝組織中NF-κB p65的錶達影響及意義。方法32隻健康雄性SD大鼠隨機分為4組，每組8隻。正常對照組：尾靜脈註射生理鹽水；陰性對照Ad-EGFP組：尾靜脈註射腺病毒包裝的增彊型綠色熒光蛋白（ Ad-EGFP）；Ad-IGFBPrP12週組：尾靜脈註射 Ad-IGFBPrP1；Ad-IGFBPrP14週組：尾靜脈註射Ad-IGFBPrP1。分彆于註射2週、4週末乙醚痳醉大鼠，取肝左葉固定、包埋、切片。行HE染色，觀察肝組織病理改變；苦味痠-天狼星紅染色觀察肝組織中膠原纖維含量的變化；免疫組織化學染色觀察IGFBPrP1、覈因子κBp65（NF-κBp65）、α-平滑肌肌動蛋白（α-SMA）、纖維連接蛋白（Fn）的錶達及分佈。結果 HE和苦味痠-天狼星紅染色顯示：與正常對照組、陰性對照Ad-EGFP組的正常肝組織相比，Ad-IGFBPrP1組肝組織齣現病理改變且髮生纖維化（ P ＜0.01）。免疫組織化學染色顯示：Ad-IGFBPrP1組肝組織中IGFBPrP1、NF-κB p65、α-SMA、Fn的錶達較正常對照組、陰性對照Ad-EGFP組明顯增彊（ P＜0.01）；Ad-IGFBPrP14週組的錶達高于2週組（ P＜0.01）。結論 Ad-IGFBPrP1經尾靜脈註射成功轉染進入大鼠，引起肝組織中NF-κB p65錶達增高，最終導緻肝組織纖維化，提示IGFBPrP1緻肝纖維化的機製之一可能通過NF-κB信號轉導通路實現。
목적：탐토선병독포장적이도소양생장인자결합단백상관단백1（ Ad-IGFBPrP1）대대서간조직중NF-κB p65적표체영향급의의。방법32지건강웅성SD대서수궤분위4조，매조8지。정상대조조：미정맥주사생리염수；음성대조Ad-EGFP조：미정맥주사선병독포장적증강형록색형광단백（ Ad-EGFP）；Ad-IGFBPrP12주조：미정맥주사 Ad-IGFBPrP1；Ad-IGFBPrP14주조：미정맥주사Ad-IGFBPrP1。분별우주사2주、4주말을미마취대서，취간좌협고정、포매、절편。행HE염색，관찰간조직병리개변；고미산-천랑성홍염색관찰간조직중효원섬유함량적변화；면역조직화학염색관찰IGFBPrP1、핵인자κBp65（NF-κBp65）、α-평활기기동단백（α-SMA）、섬유련접단백（Fn）적표체급분포。결과 HE화고미산-천랑성홍염색현시：여정상대조조、음성대조Ad-EGFP조적정상간조직상비，Ad-IGFBPrP1조간조직출현병리개변차발생섬유화（ P ＜0.01）。면역조직화학염색현시：Ad-IGFBPrP1조간조직중IGFBPrP1、NF-κB p65、α-SMA、Fn적표체교정상대조조、음성대조Ad-EGFP조명현증강（ P＜0.01）；Ad-IGFBPrP14주조적표체고우2주조（ P＜0.01）。결론 Ad-IGFBPrP1경미정맥주사성공전염진입대서，인기간조직중NF-κB p65표체증고，최종도치간조직섬유화，제시IGFBPrP1치간섬유화적궤제지일가능통과NF-κB신호전도통로실현。
Objective To investigate the expression and significance of NF-κB p65 in rat liver tissue which affected by IGFBPrP 1 mediated by adenovirus vector .Methods Thirty-two clean male 6-week-old SD rats were set into four groups randomly (8 in each).Normal control group(the same volume of normal saline was injected into caudal vein once ),egative control Ad-EGFP group(Ad-EGFP of 4 ×109 pfu was injected into caudal vein once ),Ad-IGFBPrP1 2 weeks group(Ad-IGFBPrP1 of 4 ×109 pfu was injected into caudal vein once ) ,Ad-IGFBPrP1 4 weeks group ( Ad-IGFBPrP1 of 4 ×10 9 pfu was injected into caudal vein once).Two weeks and four weeks later ,rats in each group were narcotized with diethyl ether to collect and fix left hepatic lobe .Morphological change of liver tissue was observed by HE staining .Collagen fiber content in liver tissue was detected by picric acid-Sirius red staining.Distribution and dynamic expression of IGFBPrP 1,α-SMA,NF-κB p65 and Fn in each group were detected by immunohistochemistry staining .Results 1. Comparing with normal control group and negative control Ad-EGFP group, Ad-IGFBPrP1 groups showed pathological changes and fibrosis (P<0.01).In normal control group and negative control Ad-EGFP group, the hepatic lobular structure was integrity , hepatic cords were neat , plump cytoplasm of liver cells , without inflammation,necrosis et al pathological changes .Only thin collagen fibers were visible in the vascular wall . The liver in Ad-IGFBPrP1 groups were disorder with fatty degeneration of hepatocytes , inflammatory cell infiltration and rare necrosis ,apoptosis;Collagen fibers in central vein and portal area significantly increased , collagen deposited in the liver sinusoid , fiber cord stretched between central veins .2.The contents of IGFBPrP1,NF-κB p65,α-SMA, Fn were significantly increased in Ad-IGFBPrP 1 group of2, 4 weeks comparedwith normal control group and negative control Ad E-GFP group ( P<0.01 ) .The expressions of IGFBPrP1, NF-κB p65,α-SMA,Fn in Ad-IGFBPrP1 4 weeks group were higher than those in Ad-IGFBPrP1 2 weeks group ( P<0.01 ) .Conclusions Ad-IGFBPrP1 injected into caudal vein in rats can result in the high expression of NF-κBp 65,correlated with that ofα-SMA,Fn,suggesting that NF-κB signaling pathway may be involved in the formation and development of hepatic fibrosis .
