临床麻醉学杂志
臨床痳醉學雜誌
림상마취학잡지
THE JOURNAL OF CLINICAL ANESTHESIOLOGY
2014年
7期
693-696
,共4页
雷公藤红素%Cdk5%p25%p35%阿尔茨海默病%手术创伤
雷公籐紅素%Cdk5%p25%p35%阿爾茨海默病%手術創傷
뢰공등홍소%Cdk5%p25%p35%아이자해묵병%수술창상
Celastrol%Cdk5%p25%p35%Alzheimers disease%Surgical trauma
目的：动态观察雷公藤红素对APPswe/PS1dE9双转基因阿尔茨海默病(alzheimersdisease, AD)模型小鼠肝叶部分切除术后认知功能及海马内周期蛋白依赖性蛋白激酶5(cyclin-de-pendentkinase5, Cdk5)和激动因子p25和p35表达的影响。方法3月龄AD模型小鼠96只,随机均分为三组：手术组(S组),麻醉后行肝叶部分切除术；雷公藤红素组(C组),手术前3d上午腹腔注射雷公藤红素60μg/kg；二甲亚枫(DMSO)组(O组),手术前3d上午腹腔注射同体积的0.04%DMSO。三组随机抽取8只小鼠采用水迷宫连续训练5d并检测术后1、3、7、14d的学习记忆能力,余下小鼠分别于术后1、3、7、14d取标本,每次6只,检测海马内细胞Cdk5及激动因子p25、p35的表达。结果与C组比较,术后3、7和14dS和O组小鼠逃避潜伏期明显延长(P<0.05)、目的象限时间百分比明显降低(P<0.05)；与C组比较, S和O组术后3、7、14d海马内Cdk5表达和术后1、3、7、14d海马内p25表达明显增高(P<0.01)。三组小鼠海马内p35表达差异无统计学意义。结论雷公藤红素可改善肝叶部分切除术后AD模型小鼠的认知功能的下降,其机制可能与Cdk5和p25蛋白表达下降有关。
目的：動態觀察雷公籐紅素對APPswe/PS1dE9雙轉基因阿爾茨海默病(alzheimersdisease, AD)模型小鼠肝葉部分切除術後認知功能及海馬內週期蛋白依賴性蛋白激酶5(cyclin-de-pendentkinase5, Cdk5)和激動因子p25和p35錶達的影響。方法3月齡AD模型小鼠96隻,隨機均分為三組：手術組(S組),痳醉後行肝葉部分切除術；雷公籐紅素組(C組),手術前3d上午腹腔註射雷公籐紅素60μg/kg；二甲亞楓(DMSO)組(O組),手術前3d上午腹腔註射同體積的0.04%DMSO。三組隨機抽取8隻小鼠採用水迷宮連續訓練5d併檢測術後1、3、7、14d的學習記憶能力,餘下小鼠分彆于術後1、3、7、14d取標本,每次6隻,檢測海馬內細胞Cdk5及激動因子p25、p35的錶達。結果與C組比較,術後3、7和14dS和O組小鼠逃避潛伏期明顯延長(P<0.05)、目的象限時間百分比明顯降低(P<0.05)；與C組比較, S和O組術後3、7、14d海馬內Cdk5錶達和術後1、3、7、14d海馬內p25錶達明顯增高(P<0.01)。三組小鼠海馬內p35錶達差異無統計學意義。結論雷公籐紅素可改善肝葉部分切除術後AD模型小鼠的認知功能的下降,其機製可能與Cdk5和p25蛋白錶達下降有關。
목적：동태관찰뢰공등홍소대APPswe/PS1dE9쌍전기인아이자해묵병(alzheimersdisease, AD)모형소서간협부분절제술후인지공능급해마내주기단백의뢰성단백격매5(cyclin-de-pendentkinase5, Cdk5)화격동인자p25화p35표체적영향。방법3월령AD모형소서96지,수궤균분위삼조：수술조(S조),마취후행간협부분절제술；뢰공등홍소조(C조),수술전3d상오복강주사뢰공등홍소60μg/kg；이갑아풍(DMSO)조(O조),수술전3d상오복강주사동체적적0.04%DMSO。삼조수궤추취8지소서채용수미궁련속훈련5d병검측술후1、3、7、14d적학습기억능력,여하소서분별우술후1、3、7、14d취표본,매차6지,검측해마내세포Cdk5급격동인자p25、p35적표체。결과여C조비교,술후3、7화14dS화O조소서도피잠복기명현연장(P<0.05)、목적상한시간백분비명현강저(P<0.05)；여C조비교, S화O조술후3、7、14d해마내Cdk5표체화술후1、3、7、14d해마내p25표체명현증고(P<0.01)。삼조소서해마내p35표체차이무통계학의의。결론뢰공등홍소가개선간협부분절제술후AD모형소서적인지공능적하강,기궤제가능여Cdk5화p25단백표체하강유관。
Objective To investigate the impact of celastrol on cognitive function and the ex-pression of Cdk5/p25 in hippocampus in APPswe/PS1dE9 double transgenic mouse after partial hepa-tectomy.Methods Three-month-old APPswe/PS1dE9 double transgenic mice (n=96)were randomly divided into surgery group (partial hepatectomy,group S),celastrol group (celastrol and partial hepa-tectomy,group C)and DMSO group (DMSO and partial hepatectomy,group O).Eight mice were se-lected randomly in each group and were Morris-water maze trained for 5 continuous days.Their learn-ing and memory abilities were evaluated at 1,3,7 and 14 d after the surgery,respectively.For the remaining mice in each group,the hippocampus were collected and the changes of Cdk5,p35 and p25 in hippocampus were measured by western blot at the time 1,3,7,14 d after partial hepatectomy. Six mice were killed at each time for data collection.Results The average escape latency of group C was significantly shorter than those of groups S and O at 3,7 and 14 d after partial hepatectomy (P <0.05).The percentages of time in target quadrant of groups S and O decreased significantly than that in group C (P <0.05).Western blot showed the expression of Cdk5 in group C was significantly low-er than that in groups S and O at 3,7 and 14 d after partial hepatectomy (P <0.05),and the same re-sult was also found in the expression of p25 at 1,3,7 and 14 d after partial hepatectomy (P <0.05). There was no significant difference in the expression of p35 between each group.Conclusion Celas-trol can improve the learning and memory ability in APPswe/PS1dE9 the double transgenic mouse, with the mechanism may be related to the decrease of Cdk5 and p25 in hippocampus.
