CAJ | 학술논문

目的：评价不同剂量右美托咪定预先给药对大鼠布比卡因心脏毒性的影响。方法健康雄性 SD 大鼠48只,体重250~300 g,采用随机数字表法,将其随机均分为四组：生理盐水对照组(C 组)、右美托咪定5μg/kg 组(D5组)、右美托咪定10μg/kg 组(D10组)和右美托咪定15μg/kg 组(D15组)。以肢体Ⅱ导联监测 ECG,股动脉置管监测 MAP,股静脉置管给药。D5、D10、D15组分别于布比卡因给药前15 min 时经股静脉分别泵注右美托咪定5、10和15μg/kg,C 组给予等容量生理盐水。四组大鼠均泵注0.75%布比卡因2 mg·kg-1·min-1。分别记录大鼠发生抽搐、心律失常及心脏停搏的时间和布比卡因的用量,并测定心肌组织布比卡因含量。结果与C 组比较, D5、D10和 D15组发生抽搐、心律失常和心脏停搏的时间明显延长(P <0.05),布比卡因的用量及心肌布比卡因含量明显增加(P <0.05)；与 D5组比较,D10和 D15组发生抽搐、心律失常和心脏停搏的时间明显延长,布比卡因的用量及心肌布比卡因含量明显增加(P <0.05)；D10与 D15组发生抽搐、心律失常和心脏停搏的时间、布比卡因的用量及心肌布比卡因含量差异无统计学意义。结论静脉内预先给予右美托咪定可明显提高布比卡因中毒剂量阈值,延迟布比卡因心脏毒性的发生时间,减轻大鼠布比卡因心脏毒性反应,且在一定剂量范围内呈剂量依赖性。
목적：평개불동제량우미탁미정예선급약대대서포비잡인심장독성적영향。방법건강웅성 SD 대서48지,체중250~300 g,채용수궤수자표법,장기수궤균분위사조：생리염수대조조(C 조)、우미탁미정5μg/kg 조(D5조)、우미탁미정10μg/kg 조(D10조)화우미탁미정15μg/kg 조(D15조)。이지체Ⅱ도련감측 ECG,고동맥치관감측 MAP,고정맥치관급약。D5、D10、D15조분별우포비잡인급약전15 min 시경고정맥분별빙주우미탁미정5、10화15μg/kg,C 조급여등용량생리염수。사조대서균빙주0.75%포비잡인2 mg·kg-1·min-1。분별기록대서발생추휵、심률실상급심장정박적시간화포비잡인적용량,병측정심기조직포비잡인함량。결과여C 조비교, D5、D10화 D15조발생추휵、심률실상화심장정박적시간명현연장(P <0.05),포비잡인적용량급심기포비잡인함량명현증가(P <0.05)；여 D5조비교,D10화 D15조발생추휵、심률실상화심장정박적시간명현연장,포비잡인적용량급심기포비잡인함량명현증가(P <0.05)；D10여 D15조발생추휵、심률실상화심장정박적시간、포비잡인적용량급심기포비잡인함량차이무통계학의의。결론정맥내예선급여우미탁미정가명현제고포비잡인중독제량역치,연지포비잡인심장독성적발생시간,감경대서포비잡인심장독성반응,차재일정제량범위내정제량의뢰성。
Objective To investigate the effects of different doses of dexmedetomidine pretreat-ment on cardiac toxicity of bupivacaine in rats.Methods Forty eight adult male SD rats weighing 250-300 g were randomly divided into 4 groups (n=12):saline control group (group C),dexmedetomi-dine 5 μg/kg group(group D5),dexmedetomidine 10 μg/kg group(group D10)and dexmedetomidine 1 5 μg/kg group(group D1 5 ).A Ⅱ-lead electrocardiogram(ECG)was continuously monitored,the femoral artery was cannulated for direct measurement of MAP and the femoral vein was cannulated for infusion of drugs.Groups D5,D10 and D1 5 were received infusion of dexmedetomidine 5,10 and 1 5μg/kg respectively 1 5 minutes before administration of bupivacaine,while the equal volume of saline was given in group C,then all rats received infusion 0.75% bupivacaine at the rate of 2 mg·kg-1· min-1 until asystole occurred.The doses of bupivacaine and the times of bupivacaine-induced convul-sions,arrhythmia and asystole were recorded respectively,and the myocardial concentration of bupiv-acaine was observed.Results Compared with group C,the doses of bupivacaine and the times of bupivacaine-induced convulsions,arrhythmia and asystole were all increased in groups D5,D10 and D1 5 (P <0.05).Compared with group D5,the above parameters were increased in groups D10 and D1 5 (P <0.05 ).There was no statistical significance of the above parameters between groups D10 and group D1 5.Conclusion Dexmedetomidine pretreatment can raise the threshold toxic dose of bupi-vacaine,delay the time of occurrence of cardiotoxicity of bupivacaine,so that to prevent the cardiac toxicities of bupivacaine in rats,and it produces a dose-dependent protective effect within a certain dose range.