临床麻醉学杂志
臨床痳醉學雜誌
림상마취학잡지
THE JOURNAL OF CLINICAL ANESTHESIOLOGY
2014年
7期
701-704
,共4页
吴财能%屠伟峰%马武华%曹莹
吳財能%屠偉峰%馬武華%曹瑩
오재능%도위봉%마무화%조형
糖尿病%烫伤%脂质过氧化%肿瘤坏死因子-α%舒芬太尼
糖尿病%燙傷%脂質過氧化%腫瘤壞死因子-α%舒芬太尼
당뇨병%탕상%지질과양화%종류배사인자-α%서분태니
Diabetes%Burns%Lipoperoxidation%Tumor necrosis factor-α%Sulfentanyl
目的：观察舒芬太尼预处理对烫伤糖尿病和非糖尿病大鼠心肌损伤的影响。方法取糖尿病及非糖尿病 SD 大鼠各40只,随机分为八组(n=10)：非糖尿病对照组(NS 组)、非糖尿病烫伤组(NB 组)、非糖尿病舒芬太尼预处理组(NP 组)、非糖尿病纳洛酮拮抗组(NN 组)及糖尿病对照组(DS 组)、糖尿病烫伤组(DB 组)、糖尿病舒芬太尼预处理组(DP 组)、糖尿病纳洛酮拮抗组(DN 组)。取烫伤后6 h 腹主动脉血,测血浆超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量、TNF-α、心肌肌钙蛋白 I(cTnI)水平,观察心肌组织形态学改变等。结果与 NB 组比较,NP 组血浆SOD 活性明显增加,TNF-α和 cTnI 浓度明显降低,心肌含水量明显减少(P <0.05)；DB 组 TNF-α和 cTnI 浓度明显升高,心肌含水量增加(P <0.05)。与 DB 组比较,DP 组血浆 SOD 活性明显增加, MDA、TNF-α和 cTnI 浓度明显降低,心肌含水量明显减少(P <0.05)。结论糖尿病可加重烫伤大鼠的心肌损害,舒芬太尼预处理可对其产生保护作用,其机制与减轻炎性反应和抗脂质过氧化损伤有关。
目的：觀察舒芬太尼預處理對燙傷糖尿病和非糖尿病大鼠心肌損傷的影響。方法取糖尿病及非糖尿病 SD 大鼠各40隻,隨機分為八組(n=10)：非糖尿病對照組(NS 組)、非糖尿病燙傷組(NB 組)、非糖尿病舒芬太尼預處理組(NP 組)、非糖尿病納洛酮拮抗組(NN 組)及糖尿病對照組(DS 組)、糖尿病燙傷組(DB 組)、糖尿病舒芬太尼預處理組(DP 組)、糖尿病納洛酮拮抗組(DN 組)。取燙傷後6 h 腹主動脈血,測血漿超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量、TNF-α、心肌肌鈣蛋白 I(cTnI)水平,觀察心肌組織形態學改變等。結果與 NB 組比較,NP 組血漿SOD 活性明顯增加,TNF-α和 cTnI 濃度明顯降低,心肌含水量明顯減少(P <0.05)；DB 組 TNF-α和 cTnI 濃度明顯升高,心肌含水量增加(P <0.05)。與 DB 組比較,DP 組血漿 SOD 活性明顯增加, MDA、TNF-α和 cTnI 濃度明顯降低,心肌含水量明顯減少(P <0.05)。結論糖尿病可加重燙傷大鼠的心肌損害,舒芬太尼預處理可對其產生保護作用,其機製與減輕炎性反應和抗脂質過氧化損傷有關。
목적：관찰서분태니예처리대탕상당뇨병화비당뇨병대서심기손상적영향。방법취당뇨병급비당뇨병 SD 대서각40지,수궤분위팔조(n=10)：비당뇨병대조조(NS 조)、비당뇨병탕상조(NB 조)、비당뇨병서분태니예처리조(NP 조)、비당뇨병납락동길항조(NN 조)급당뇨병대조조(DS 조)、당뇨병탕상조(DB 조)、당뇨병서분태니예처리조(DP 조)、당뇨병납락동길항조(DN 조)。취탕상후6 h 복주동맥혈,측혈장초양화물기화매(SOD)활성、병이철(MDA)함량、TNF-α、심기기개단백 I(cTnI)수평,관찰심기조직형태학개변등。결과여 NB 조비교,NP 조혈장SOD 활성명현증가,TNF-α화 cTnI 농도명현강저,심기함수량명현감소(P <0.05)；DB 조 TNF-α화 cTnI 농도명현승고,심기함수량증가(P <0.05)。여 DB 조비교,DP 조혈장 SOD 활성명현증가, MDA、TNF-α화 cTnI 농도명현강저,심기함수량명현감소(P <0.05)。결론당뇨병가가중탕상대서적심기손해,서분태니예처리가대기산생보호작용,기궤제여감경염성반응화항지질과양화손상유관。
Objective To explore effects of sulfentanyl preconditioning on myocardial injury in scald in diabetic and non-diabetic rats.Methods Eighty SD rats (40 diabetic rats and 40 non-diabetic rats)were divided into eight groups (10 rats per each),including sham group(group NS,non-diabetic rats with sham burn),burned group(group NB,non-diabetic rats with third-degree burns over 30%total body surface area (TBSA)and lactated Ringer??s solution for resuscitation),sulfentanyl group (group NP,non-diabetic rats without given sulfentanyl before burning and lactated Ringer??s solution for resuscitation)and naloxone group(group NN,non-diabetic rats given naloxone before sulfentanyl group),Diabetes sham group(group DS,diabetic rats with sham burn),Diabetic rats burned group (group DB,diabetic rats given third-degree burns,over 30 percent of the total body surface area had been burned and given lactated Ringer??s solution for resuscitation),diabetic sulfentanyl group(group DP,diabetic rats given sulfentanyl before burning and given lactated Ringer??s solution for resuscita-tion)and diabetic naloxone group(group DN,diabetic rats given naloxone,after that treated as the sulfentanyl group).Results Compared to group NB,for the mice in group NP,the activity of plasma SOD increased significantly,TNF-α,cTnI and water content level in myocardium decreased signifi-cantly (P <0.05 );whereas TNF-α,cTnI and water content level in myocardium in group DB in-creased significantly (P <0.05);Compared to group DB,for the mice in group DP,the activity of plasma SOD increased significantly,MDA,TNF-α,cTnI and water content level in myocardium de-creased significantly (P <0.05).Conclusion Diabetes may deteriorate burn-induced myocardial injury in rats.Sulfentanyl pretreatment exhibits significant protective effects on burned-induced myocardial injury in severely burned diabetic rats via inhibiting lipid peroxidation and TNF-αexpression.
