西南林业大学学报
西南林業大學學報
서남임업대학학보
JOURNAL OF SOUTHWEST FORESTRY COLLEGE
2014年
4期
20-25
,共6页
王子佳%姜静%刘桂丰%刘菲菲%李慧玉
王子佳%薑靜%劉桂豐%劉菲菲%李慧玉
왕자가%강정%류계봉%류비비%리혜옥
白桦%FLC%LFY%SOC1%启动子分析%基因表达
白樺%FLC%LFY%SOC1%啟動子分析%基因錶達
백화%FLC%LFY%SOC1%계동자분석%기인표체
Betula platyphylla%FLC%LFY%SOC1%analysis of promoter%gene expression
为研究LFY、FLC及SOC1开花调控关键节点基因在白桦花时调控中的作用，从白桦转录组数据中获得了LFY和FLC的全长cDNA序列，分别命名为BpLFY和BpFLC。获得的BpLFY基因编码区长1215 bp，编码405个氨基酸，分子量为45.3 kD。BpFLC基因cDNA编码区长627 bp，编码209个氨基酸，分子量为11.4 kD，这2个基因分别属于FLO-LFY和MADS超家族。对3个基因的启动子进行分析，发现3个基因启动子区均含有2个花粉特异表达的顺式作用元件。通过实时定量PCR对BpLFY、BpFLC及Bp-SOC1在白桦茎尖、叶片、雄花序和叶腋中表达模式的分析结果表明，BpSOC1和BpFLC在白桦各个组织部位均表达，而BpLFY只在雄花序和发育中的腋芽中表达，且在叶片中BpFLC可抑制BpSOC1的表达。
為研究LFY、FLC及SOC1開花調控關鍵節點基因在白樺花時調控中的作用，從白樺轉錄組數據中穫得瞭LFY和FLC的全長cDNA序列，分彆命名為BpLFY和BpFLC。穫得的BpLFY基因編碼區長1215 bp，編碼405箇氨基痠，分子量為45.3 kD。BpFLC基因cDNA編碼區長627 bp，編碼209箇氨基痠，分子量為11.4 kD，這2箇基因分彆屬于FLO-LFY和MADS超傢族。對3箇基因的啟動子進行分析，髮現3箇基因啟動子區均含有2箇花粉特異錶達的順式作用元件。通過實時定量PCR對BpLFY、BpFLC及Bp-SOC1在白樺莖尖、葉片、雄花序和葉腋中錶達模式的分析結果錶明，BpSOC1和BpFLC在白樺各箇組織部位均錶達，而BpLFY隻在雄花序和髮育中的腋芽中錶達，且在葉片中BpFLC可抑製BpSOC1的錶達。
위연구LFY、FLC급SOC1개화조공관건절점기인재백화화시조공중적작용，종백화전록조수거중획득료LFY화FLC적전장cDNA서렬，분별명명위BpLFY화BpFLC。획득적BpLFY기인편마구장1215 bp，편마405개안기산，분자량위45.3 kD。BpFLC기인cDNA편마구장627 bp，편마209개안기산，분자량위11.4 kD，저2개기인분별속우FLO-LFY화MADS초가족。대3개기인적계동자진행분석，발현3개기인계동자구균함유2개화분특이표체적순식작용원건。통과실시정량PCR대BpLFY、BpFLC급Bp-SOC1재백화경첨、협편、웅화서화협액중표체모식적분석결과표명，BpSOC1화BpFLC재백화각개조직부위균표체，이BpLFY지재웅화서화발육중적액아중표체，차재협편중BpFLC가억제BpSOC1적표체。
LFY,FLC and SOC1 genes are key node genes in flowering regulation network.To characterize their function in birch,complete cDNA sequences of LFY and FLC were obtained from transcriptome datas of Betula platyphylla,named BpLFY and BpLFY,respectively.BpLFY was 1 215 bp in length,encoding a protein of 405 a-mino acid residues with the molecular mass of 45 .3 kD.The length of BpLFY gene cDNA was 627 bp,encoding 209 amino acid residues with the molecular mass of 1 1.4 kD,the two genes belonged to FLO-LFY and MADS su-perfamily,respectively.Analysis of promoters of these genes showed that promoters of all these genes contained two cis-acting elements of pollen specific expression.In different tissues (shoot tip,leaf,male inflorescence and axilla-ry),gene expression of BpLFY,BpFLC and BpSOC1 genes were investigated by using real-time quantitative RT-PCR.The results showed that BpSOC1 and BpFLC were expressed in various tissues of birch,however,BpLFY was expressed only in the male inflorescence and axillary,and BpFLC can inhibit BpSOC1 expression in the leaves.
