广西植物
廣西植物
엄서식물
GUIHAIA
2014年
4期
455-459
,共5页
吴柳杰%张永先%凌桂芝%黎晓峰
吳柳傑%張永先%凌桂芝%黎曉峰
오류걸%장영선%릉계지%려효봉
铝%根尖%有机酸%分泌
鋁%根尖%有機痠%分泌
려%근첨%유궤산%분비
Aluminum%root apex%organic acids%secretion
通过建立的活体根培养及微量根尖分泌物收集系统,对铝胁迫下黑麦和小麦根尖分泌的有机酸进行研究。结果表明：50、100、200、300μmol??L-1 AlCl3处理后黑麦根尖分泌柠檬酸和苹果酸,而铝仅诱导小麦根尖分泌苹果酸。铝处理3 h 后,根尖分泌的苹果酸显著增加,并在9 h 内维持较高的分泌速率。铝诱导黑麦根尖分泌柠檬酸有明显的迟缓期,Al(300μmol??L-1)处理后的最初3 h,根尖分泌的柠檬酸并不显著增加。在铝溶液中添加的阴离子通道抑制剂 A-9-C(20、60、100μmol??L-1)显著抑制根尖分泌有机酸。然而,将黑麦根尖浸泡于含异三聚体 G 蛋白激活剂霍乱毒素(50 ng??mL-1)后,根尖分泌的有机酸显著增加。说明建立的微量根尖分泌物收集系统适合于铝诱导根尖分泌有机酸的研究,小麦和黑麦根尖在铝胁迫下以不同模式通过阴离子通道分泌有机酸,而异三聚体 G 蛋白可能介导根尖分泌有机酸。
通過建立的活體根培養及微量根尖分泌物收集繫統,對鋁脅迫下黑麥和小麥根尖分泌的有機痠進行研究。結果錶明：50、100、200、300μmol??L-1 AlCl3處理後黑麥根尖分泌檸檬痠和蘋果痠,而鋁僅誘導小麥根尖分泌蘋果痠。鋁處理3 h 後,根尖分泌的蘋果痠顯著增加,併在9 h 內維持較高的分泌速率。鋁誘導黑麥根尖分泌檸檬痠有明顯的遲緩期,Al(300μmol??L-1)處理後的最初3 h,根尖分泌的檸檬痠併不顯著增加。在鋁溶液中添加的陰離子通道抑製劑 A-9-C(20、60、100μmol??L-1)顯著抑製根尖分泌有機痠。然而,將黑麥根尖浸泡于含異三聚體 G 蛋白激活劑霍亂毒素(50 ng??mL-1)後,根尖分泌的有機痠顯著增加。說明建立的微量根尖分泌物收集繫統適閤于鋁誘導根尖分泌有機痠的研究,小麥和黑麥根尖在鋁脅迫下以不同模式通過陰離子通道分泌有機痠,而異三聚體 G 蛋白可能介導根尖分泌有機痠。
통과건립적활체근배양급미량근첨분비물수집계통,대려협박하흑맥화소맥근첨분비적유궤산진행연구。결과표명：50、100、200、300μmol??L-1 AlCl3처리후흑맥근첨분비저몽산화평과산,이려부유도소맥근첨분비평과산。려처리3 h 후,근첨분비적평과산현저증가,병재9 h 내유지교고적분비속솔。려유도흑맥근첨분비저몽산유명현적지완기,Al(300μmol??L-1)처리후적최초3 h,근첨분비적저몽산병불현저증가。재려용액중첨가적음리자통도억제제 A-9-C(20、60、100μmol??L-1)현저억제근첨분비유궤산。연이,장흑맥근첨침포우함이삼취체 G 단백격활제곽란독소(50 ng??mL-1)후,근첨분비적유궤산현저증가。설명건립적미량근첨분비물수집계통괄합우려유도근첨분비유궤산적연구,소맥화흑맥근첨재려협박하이불동모식통과음리자통도분비유궤산,이이삼취체 G 단백가능개도근첨분비유궤산。
Secretion of organic acids from roots apices in rye and wheat were investigated by a system for in vivo root apex culture and micro-exudates collection,to elucidate the mechanisms for Al-induced secretion of organic acids.The results showed that Al (50,100,200,300 μmol??L-1 AlCl3 )induced secretion of citrate and malate from rye root api-ces,while wheat root apices secreted malate alone in response to Al treatment.Malate was significantly secreted from root apices of rye and wheat after the treatment with Al for 3 h,and malate was secreted with a high secretion rate within 9 h treatment.There was a lag between the secretion of citrate and Al treatment in rye.At initial 3 h,Al failed to induce significantly secretion of citrate.On the other hand,the addiction of anion channel inhibitor A-9-C (20、60、100 μmol??L-1 )to Al solution,the secretion of organic acids was significantly inhibited.However,the amount of or-ganic acids secreted elevated significantly after the exposure of root apices to Al solution with heterotrimeric G-protein agonist cholera toxin (50 ng??mL-1 ).These results suggested that our system for in vivo root apex culture and mi-cro-exudates collection was a available tool for the studies on Al-induced secretion of organic acids,and organic acids were secreted from root apices of rye and wheat by anion channels in different patterns,while heterotrimeric G-protein may mediate the secretion of organic acids from root apices.
