CAJ | 학술논문

利用建兰转录组数据对其微卫星序列又称简单重复序列( simple sequence repeat ,SSR)和单核苷酸多态性( single nucleotide polymorphism ,SNP)进行搜索,并对其所在序列进行注释,从而为建兰分子标记的开发提供有效信息.利用 High-Seq技术对建兰转录组进行深度测序,采取从头拼接策略进行拼接,最后得到了101423个转录产物,其中含139385689 bp ,平均长度为1374 bp .在这个转录组数据库中一共检测到17793个SSR和16676个SNP位点,它们的平均密度分别是1.28个SSRs/10 kb和1.20个SNPs/10 kb .在这些SSR中,除了单核苷酸重复外,二核苷酸和三核苷酸重复是最主流的类型,分别占了所有SSR的20.46％和21.98％.在SNP中,C和 T之间以及A和G之间的替换是最主要的形式,分别占了所有SNP的30.80％和28.81％.另外,对含有SSR和SNP序列注释发现：分别有1748个SSR和1932个SNP序列具有直系同源基因簇( Clusters of Orthologous Groups ,COG)注释；4994个SSR和4819个SNP序列具有基因本体论(Gene Ontology ,GO)注释；2107个SSR和2188个SNP序列具有京都基因与基因组百科( Kyoto Encyclopedia of Genes and Genomes ,KEGG)注释.这些序列涉及了许多重要的生物功能和代谢途径,预示着这些潜在的标记可能与重要的生物功能有关.这些信息为建兰分子标记的开发和应用奠定了基础.
이용건란전록조수거대기미위성서렬우칭간단중복서렬( simple sequence repeat ,SSR)화단핵감산다태성( single nucleotide polymorphism ,SNP)진행수색,병대기소재서렬진행주석,종이위건란분자표기적개발제공유효신식.이용 High-Seq기술대건란전록조진행심도측서,채취종두병접책략진행병접,최후득도료101423개전록산물,기중함139385689 bp ,평균장도위1374 bp .재저개전록조수거고중일공검측도17793개SSR화16676개SNP위점,타문적평균밀도분별시1.28개SSRs/10 kb화1.20개SNPs/10 kb .재저사SSR중,제료단핵감산중복외,이핵감산화삼핵감산중복시최주류적류형,분별점료소유SSR적20.46％화21.98％.재SNP중,C화 T지간이급A화G지간적체환시최주요적형식,분별점료소유SNP적30.80％화28.81％.령외,대함유SSR화SNP서렬주석발현：분별유1748개SSR화1932개SNP서렬구유직계동원기인족( Clusters of Orthologous Groups ,COG)주석；4994개SSR화4819개SNP서렬구유기인본체론(Gene Ontology ,GO)주석；2107개SSR화2188개SNP서렬구유경도기인여기인조백과( Kyoto Encyclopedia of Genes and Genomes ,KEGG)주석.저사서렬섭급료허다중요적생물공능화대사도경,예시착저사잠재적표기가능여중요적생물공능유관.저사신식위건란분자표기적개발화응용전정료기출.
Summary Cymbidium ensi f olium is one of Cymbidium genus , having elegant shape , beautiful appearance and fragrant aroma . Because of these features , this species gets with extremely high ornamental value . Owing to the lack of its genomic resource , the development and application of molecular marker is still limited . With the development of RNA-Seq technology , the transcriptomic data gradually accumulate and become a useful resource to explore marker with low cost and high efficiency .