南昌大学学报(理科版)
南昌大學學報(理科版)
남창대학학보(이과판)
JOURNAL OF NANCHANG UNIVERSITY(NATURAL SCIENCE)
2014年
3期
222-227
,共6页
徐慧%刘伟%刘伟%刘成梅%王炎炎%周磊
徐慧%劉偉%劉偉%劉成梅%王炎炎%週磊
서혜%류위%류위%류성매%왕염염%주뢰
酱鸭%质构%蛋白降解%电泳
醬鴨%質構%蛋白降解%電泳
장압%질구%단백강해%전영
sauced duck%texture%proteolysis%electrophoresis
酱鸭加工过程(盐腌、酱制、收汁、灭菌)中质构特性(硬度、弹性)和肌肉蛋白(肌浆蛋白、肌原纤维蛋白)降解的变化规律。结果表明:在盐腌过程中,鸭肉硬度呈现先升高后降低趋势,盐腌6 h硬度最高,12 h硬度最低;盐腌过程中的弹性总体呈下降趋势,腌制9h后弹性最低。酱制、收汁、灭菌过程鸭肉硬度明显高于盐腌过程,在酱制阶段,硬度变化不显著,经过灭菌后的酱鸭硬度有所下降;该过程中的鸭肉弹性低于盐腌过程,变化不显著。SDS-PAGE电泳分析显示:在盐腌阶段,鸭肉肌原纤维蛋白中的α-肌动蛋白素条带比较稳定,其余的肌原纤维蛋白和肌浆蛋白均发生着不同程度的降解;而经过酱制、收汁、灭菌后,肌原纤维蛋白条带变少,肌浆蛋白中原先的蛋白条带消失,出现了一些新的蛋白条带。从盐腌开始到酱制结束,非蛋白氮含量下降,灭菌后含量增加;鸭肉重量在加工过程中下降,特别是经过0.5 h的酱制后,鸭肉重量显著降低。
醬鴨加工過程(鹽醃、醬製、收汁、滅菌)中質構特性(硬度、彈性)和肌肉蛋白(肌漿蛋白、肌原纖維蛋白)降解的變化規律。結果錶明:在鹽醃過程中,鴨肉硬度呈現先升高後降低趨勢,鹽醃6 h硬度最高,12 h硬度最低;鹽醃過程中的彈性總體呈下降趨勢,醃製9h後彈性最低。醬製、收汁、滅菌過程鴨肉硬度明顯高于鹽醃過程,在醬製階段,硬度變化不顯著,經過滅菌後的醬鴨硬度有所下降;該過程中的鴨肉彈性低于鹽醃過程,變化不顯著。SDS-PAGE電泳分析顯示:在鹽醃階段,鴨肉肌原纖維蛋白中的α-肌動蛋白素條帶比較穩定,其餘的肌原纖維蛋白和肌漿蛋白均髮生著不同程度的降解;而經過醬製、收汁、滅菌後,肌原纖維蛋白條帶變少,肌漿蛋白中原先的蛋白條帶消失,齣現瞭一些新的蛋白條帶。從鹽醃開始到醬製結束,非蛋白氮含量下降,滅菌後含量增加;鴨肉重量在加工過程中下降,特彆是經過0.5 h的醬製後,鴨肉重量顯著降低。
장압가공과정(염업、장제、수즙、멸균)중질구특성(경도、탄성)화기육단백(기장단백、기원섬유단백)강해적변화규률。결과표명:재염업과정중,압육경도정현선승고후강저추세,염업6 h경도최고,12 h경도최저;염업과정중적탄성총체정하강추세,업제9h후탄성최저。장제、수즙、멸균과정압육경도명현고우염업과정,재장제계단,경도변화불현저,경과멸균후적장압경도유소하강;해과정중적압육탄성저우염업과정,변화불현저。SDS-PAGE전영분석현시:재염업계단,압육기원섬유단백중적α-기동단백소조대비교은정,기여적기원섬유단백화기장단백균발생착불동정도적강해;이경과장제、수즙、멸균후,기원섬유단백조대변소,기장단백중원선적단백조대소실,출현료일사신적단백조대。종염업개시도장제결속,비단백담함량하강,멸균후함량증가;압육중량재가공과정중하강,특별시경과0.5 h적장제후,압육중량현저강저。
The changes of texture (hardness and elasticity)and degradation of muscle proteins (sarcoplas-mic and myofibrillar proteins)during processes (salting,saucing,juice absorbing and sterilization)of sauced duck were studied.During the salting process,the hardness of duck meat increased firstly then de-creased with the highest hardness achieved at 6h and the lowest one at 12 h.Generally,the elasticity de-creased during the salting process,down to the lowest level at 9 h.The hardness at saucing process,j uice absorbing and sterilization were higher than that at salting process.The hardness did not change obviously during the saucing process,but it decreased after sterilizing.The elasticity at saucing,j uice absorbing and sterilization were lower than that at salting process with no significant change SDS-PAGE results revealed thatα-actinin was stable while other myofibrillar proteins and sarcoplasmic proteins were degraded during the salting process.After saucing,j uice absorbing and sterilization,bands of myofibrillar proteins became less,and bands of sarcoplasmic protein disappeared,however,some new bands appeared.From the salting processing to the end of saucing,non-protein nitrogen decreased,while it increased after sterilization.The weight of duck meat decreased during the whole process,especially after 0.5h saucing.