CAJ | 학술논문

建立了一种大鼠尿液中黄蝶呤、异黄蝶呤、酪氨酸和色氨酸的超高效液相色谱-荧光分析方法（UPLC-FLD），大鼠尿液利用甲醇和乙腈除蛋白后，采取C18固相萃取小柱进行净化，然后用UPLC-FLD法进行测定。黄蝶呤、异黄蝶呤、酪氨酸和色氨酸分别在0．01～10．0，0．005～10．0，0．5～100，0．5～200 mg·L-1范围内与色谱峰面积呈线性关系，4种物质的仪器检测限分别为0．005，0．0003，0．004和0．002 mg·L-1，加标回收率除黄蝶呤在61．1％～78．0％之间，其余各物质回收率均在86．0％～110．0％之间，RSD小于10．5％。
건립료일충대서뇨액중황접령、이황접령、락안산화색안산적초고효액상색보-형광분석방법（UPLC-FLD），대서뇨액이용갑순화을정제단백후，채취C18고상췌취소주진행정화，연후용UPLC-FLD법진행측정。황접령、이황접령、락안산화색안산분별재0．01～10．0，0．005～10．0，0．5～100，0．5～200 mg·L-1범위내여색보봉면적정선성관계，4충물질적의기검측한분별위0．005，0．0003，0．004화0．002 mg·L-1，가표회수솔제황접령재61．1％～78．0％지간，기여각물질회수솔균재86．0％～110．0％지간，RSD소우10．5％。
A simple,rapid and sensitive method for the determination of xanthopterin,isoxanthopterin,tyro-sine and tryptophan in mouse urine by UPLC-FLD was proposed in this study.After deproteinized with methanol and acetonitrile,the mouse urine samples were purified with C1 8 solid phase extraction column and then detected directly by UPLC-FLD.Xanthopterin,isoxanthopterin,tyrosine and tryptophan behaved linearly in the range of 0.01~10.0 mg/L,0.005~10.0 mg·L-1 ,0.5~100 mg·L-1 and 0.5~200 mg· L-1 ,respectively.The limits of instrument detection of the four analytes were found to be 0.005、0.0003、0.004 and 0.002 mg·L-1 ,respectively.The recoveries were in the range of 86.0% ~ 110.0% except that of xanthopterin were in the range of 61.1% ~78.0%.The relative standard deviations were lower than 10.5%.