CAJ | 학술논문

建立同时测定阿糖胞苷和代谢产物阿糖尿苷在大鼠血浆中浓度的 LC-MS/MS方法，并将该方法用于研究阿糖胞苷静脉注射后血药浓度的监测。色谱柱是安捷伦ZORBAX SB-C18色谱柱（50 mm×2．1 mm，1．8μm），采用梯度洗脱的方式分离被测物质，利用沉淀蛋白的方法处理血浆样品，以多反应监测（multiple reaction monitoring， MRM）扫描方式检测。阿糖胞苷和阿糖尿苷在线性范围内线性关系良好，日内日间精密度（RSD）均小于15％，准确度（RE）在±15％以内，平均提取回收率都在80％以上，基质效应在90％和110％之间。该方法适用于阿糖胞苷注射后的临床前药物监测和大鼠药物动力学研究。
건립동시측정아당포감화대사산물아당뇨감재대서혈장중농도적 LC-MS/MS방법，병장해방법용우연구아당포감정맥주사후혈약농도적감측。색보주시안첩륜ZORBAX SB-C18색보주（50 mm×2．1 mm，1．8μm），채용제도세탈적방식분리피측물질，이용침정단백적방법처리혈장양품，이다반응감측（multiple reaction monitoring， MRM）소묘방식검측。아당포감화아당뇨감재선성범위내선성관계량호，일내일간정밀도（RSD）균소우15％，준학도（RE）재±15％이내，평균제취회수솔도재80％이상，기질효응재90％화110％지간。해방법괄용우아당포감주사후적림상전약물감측화대서약물동역학연구。
to develop a HPLC-MS/MS method to determine the concentration of ara-C and ara-U in rat plas-ma,and apply this method to the pharmacokinetics study and therapeutic drug monitoring after an inj ection of ara-C to rats.Method:The analytes were separated on a C18 column(50 × 2.1 mm,1.8μm)and a tri-ple-quadrupole mass spectrometry equipped with an electrospray ionization(ESI)source was applied for de-tection.The plasma sample was prepared by protein precipitation method.Result:The calibration curves were linear over a concentration range of 2.1-2100.0 ng·mL-1 for ara-C and 2.08-2080.0 ng·mL-1 for ara-U.The intra-day and inter-day precision was less than 15% and the relative errors(RE)were all within±15%.The recovery for all analytes was over 80%.Conclusion:The validated method was success-fully applied to a preclinical pharmacokinetics study and the therapeutic drug monitoring after the i.v.ad-ministration of ara-C to rats.