国际沙棘研究与开发
國際沙棘研究與開髮
국제사극연구여개발
THE GLOBAL SEABUCKTHORN RESEARCH AND DEVELOPMENT
2014年
2期
22-24,34
,共4页
沙棘黄酮粉%含量测定%HPLC
沙棘黃酮粉%含量測定%HPLC
사극황동분%함량측정%HPLC
seabuckthorn flavone powder%content determination%HPLC
建立同时测定沙棘黄酮粉中槲皮素、山柰素和异鼠李素三种成分含量的HPLC方法,对收集到的7个批次沙棘黄酮粉样品进行质量分析。采用 Kromasil C18(4.6mm ×250mm ,5μm)色谱柱为固定相,甲醇-0.4%磷酸水溶液为流动相,进行梯度洗脱,流速1.0mL/min ,检测波长为370nm ,柱温25℃。结果显示:该方法稳定可靠,重复性强。收集到的7个批次的沙棘黄酮粉样品中三种成分的含量差异较大,即便是同一厂家不同批次的产品差异也较大。
建立同時測定沙棘黃酮粉中槲皮素、山柰素和異鼠李素三種成分含量的HPLC方法,對收集到的7箇批次沙棘黃酮粉樣品進行質量分析。採用 Kromasil C18(4.6mm ×250mm ,5μm)色譜柱為固定相,甲醇-0.4%燐痠水溶液為流動相,進行梯度洗脫,流速1.0mL/min ,檢測波長為370nm ,柱溫25℃。結果顯示:該方法穩定可靠,重複性彊。收集到的7箇批次的沙棘黃酮粉樣品中三種成分的含量差異較大,即便是同一廠傢不同批次的產品差異也較大。
건립동시측정사극황동분중곡피소、산내소화이서리소삼충성분함량적HPLC방법,대수집도적7개비차사극황동분양품진행질량분석。채용 Kromasil C18(4.6mm ×250mm ,5μm)색보주위고정상,갑순-0.4%린산수용액위류동상,진행제도세탈,류속1.0mL/min ,검측파장위370nm ,주온25℃。결과현시:해방법은정가고,중복성강。수집도적7개비차적사극황동분양품중삼충성분적함량차이교대,즉편시동일엄가불동비차적산품차이야교대。
Objective:To set up a RP-HPLC method to determine the content of Quercetin, Kaempfer-ide and Isorhamnetin in Seabuckthorn flavone powder. Method:The optimum HPLC condition was as follows:Kromasil C18 column (4.6mm × 250mm, 5μm)as the stationary phase;Mobile Phase:Metha-nol-0.4% Phosphoric acid, gradient elution, detection wavelength:370nm;Column Temperature:25℃;Flow Rate:1.0mL/min. Results:The HPLC method was reasonable, stable and accurate. Conclusion:The contents of three components in seven batchs of seabuckthorn flavone powder we collected was quite difference, even in different batchs producted by same company.