生物技术进展
生物技術進展
생물기술진전
2014年
4期
292-295
,共4页
周艳%张涛%郑勤琴%杨承霖%樊皓月
週豔%張濤%鄭勤琴%楊承霖%樊皓月
주염%장도%정근금%양승림%번호월
连翘%成分提取%奶牛乳房炎%致病菌%抑制作用
連翹%成分提取%奶牛乳房炎%緻病菌%抑製作用
련교%성분제취%내우유방염%치병균%억제작용
forsythia suspense%component extraction%cow mastitis%pathogens%inhibition
中药连翘具有抗菌、消炎、解热等作用，本研究目的在于摸索连翘有效成分提取的试验方法，获得适于抑制奶牛乳房炎致病菌的有效成分。以连翘叶片为材料，利用回流法提取工艺提取，采用薄层色谱（ TLC）测定法定性分析，高效液相色谱法（ HPLC）测定有效成分纯度，抑菌圈检测法检测抑菌效果。结果表明，连翘提取物对奶牛乳房炎致病菌具有抑制效果。
中藥連翹具有抗菌、消炎、解熱等作用，本研究目的在于摸索連翹有效成分提取的試驗方法，穫得適于抑製奶牛乳房炎緻病菌的有效成分。以連翹葉片為材料，利用迴流法提取工藝提取，採用薄層色譜（ TLC）測定法定性分析，高效液相色譜法（ HPLC）測定有效成分純度，抑菌圈檢測法檢測抑菌效果。結果錶明，連翹提取物對奶牛乳房炎緻病菌具有抑製效果。
중약련교구유항균、소염、해열등작용，본연구목적재우모색련교유효성분제취적시험방법，획득괄우억제내우유방염치병균적유효성분。이련교협편위재료，이용회류법제취공예제취，채용박층색보（ TLC）측정법정성분석，고효액상색보법（ HPLC）측정유효성분순도，억균권검측법검측억균효과。결과표명，련교제취물대내우유방염치병균구유억제효과。
Forsythia suspense has the properties of anti-bacteria, anti-inflammatory, antipyretic and other features. The purpose of this study is to explore the test method of effective component exraction from Chinese medicine Forsythia and explore the component for pathogen inhibiton in cow mastitis. The Forsythia leaves were extracted using reflux extraction technology, the extract was analyzed by TLC, and the purity of the extracted active component was detected by HPLC. Antimicrobial effects of Forsythia suspensa extract was detected using inhibition zone assay. The results showed that extract of Forsythia suspensa had inhibitory effect on dairy cow mastitis pathogens.
