CAJ | 학술논문

油菜素内酯（brassinosteroid， BRs）是一类重要的植物激素，在植物的生长发育过程中发挥重要的调节作用。BRs的信号转导研究在双子叶植物拟南芥中已取得重大进展，但在单子叶植物水稻中， BRs的信号转导途径尚不很清楚。本研究从水稻T-DNA插入突变体库中筛选出一个叶片直立突变体el（ erect leave mutant）。该突变体与野生型植株相比，叶夹角减小。遗传分析显示，el的突变性状由一对显性基因控制。该基因经图位克隆定位于水稻第5染色体引物InDel3和InDel4之间，物理距离为700 kb。本研究明确了一个水稻BRs不敏感突变体的表型特征及遗传规律，为进一步研究水稻BRs信号转导调控机制奠定基础。
유채소내지（brassinosteroid， BRs）시일류중요적식물격소，재식물적생장발육과정중발휘중요적조절작용。BRs적신호전도연구재쌍자협식물의남개중이취득중대진전，단재단자협식물수도중， BRs적신호전도도경상불흔청초。본연구종수도T-DNA삽입돌변체고중사선출일개협편직립돌변체el（ erect leave mutant）。해돌변체여야생형식주상비，협협각감소。유전분석현시，el적돌변성상유일대현성기인공제。해기인경도위극륭정위우수도제5염색체인물InDel3화InDel4지간，물리거리위700 kb。본연구명학료일개수도BRs불민감돌변체적표형특정급유전규률，위진일보연구수도BRs신호전도조공궤제전정기출。
Brassinosteroids ( BRs) are a kind of essential hormones that play important roles in plant growth and development. In Arabidopsis, many components of the BRs signaling pathway have been identified and studied in detail. However, some key components functioning in BRs signaling have not been described in rice. In this study, we identified an erect leave mutant from our rice mutant library, designated as el ( erect leave mutant) . Compared with wild-type plants, el exhibited erect leaves. Further physiological experiments showed that el is insensitive to exogenous BRs. Genetic analysis showed that the mutant is controlled by a dominant gene. The map-based cloning results showed that the mutant locus was located on rice chromosome 5 between primers InDel3 and InDel4, the physical distance is about 700 kb. In this study, we identified a new BRs insensitive mutant in rice, which will be an important material to uncover a new component in rice BRs signaling.